State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China; Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, PR China.
Bioresour Technol. 2017 Dec;245(Pt B):1498-1506. doi: 10.1016/j.biortech.2017.05.142. Epub 2017 Jun 12.
Biofuels, including ethanol and butanol, are mainly produced by mesophilic solventogenic yeasts and Clostridium species. However, these microorganisms cannot directly utilize lignocellulosic materials, which are abundant, renewable and non-compete with human demand. More recently, thermophilic bacteria show great potential for biofuels production, which could efficiently degrade lignocellulose through the cost effective consolidated bioprocessing. Especially, it could avoid contamination in the whole process owing to its relatively high fermentation temperature. However, wild types thermophiles generally produce low levels of biofuels, hindering their large scale production. This review comprehensively summarizes the state of the art development of biofuels production by reported thermophilic microorganisms, and also concludes strategies to improve biofuels production including the metabolic pathways construction, co-culturing systems and biofuels tolerance. In addition, strategies to further improve butanol production are proposed.
生物燃料,包括乙醇和丁醇,主要由嗜温溶剂产生菌酵母和梭菌属微生物生产。然而,这些微生物不能直接利用丰富、可再生且不与人争粮的木质纤维素材料。最近,嗜热细菌在生物燃料生产方面显示出巨大的潜力,通过具有成本效益的整合生物加工,它们可以有效地降解木质纤维素。特别是,由于其相对较高的发酵温度,可以避免整个过程中的污染。然而,野生型嗜热菌通常生物燃料产量较低,限制了其大规模生产。本综述全面总结了报道的嗜热微生物生产生物燃料的最新技术进展,并总结了提高生物燃料生产的策略,包括代谢途径构建、共培养系统和生物燃料耐受性。此外,还提出了进一步提高丁醇产量的策略。