（紫葳科）洛曼氏（紫葳属）提取物及其成分的抗病毒活性
Antiviral Activity of (Bureau) L. G. Lohmann (Bignoniaceae) Extracts and Constituents.
作者信息
Brandão Geraldo Célio, Kroon Erna G, Souza Filho José D, Oliveira Alaíde Braga
机构信息
Departamento de Farmácia, Escola de Farmácia, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, 35.400-000 Ouro Preto, MG, Brazil.
Departamento de Microbiologia, ICB, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, 31.270-901 Belo Horizonte, MG, Brazil.
出版信息
J Trop Med. 2017;2017:6106959. doi: 10.1155/2017/6106959. Epub 2017 May 29.
A phytochemical study of (Bignoniaceae) ethanol extracts of leaves, stems, and fruits was guided by in vitro assays against vaccinia virus Western Reserve (VACV-WR), human herpes virus 1 (HSV-1), murine encephalomyocarditis virus (EMCV), and dengue virus type 2 (DENV-2) by the MTT method. All the ethanol extracts were active against DENV-2, HSV-1, and VACV-WR with best results for the fruits extract against DENV-2 (SI > 38.2). For VACV-WR and HSV-1, EC50 values > 200 g mL were determined, while no inhibition of the cytopathic effect was observed with EMCV. Five compounds were isolated and identified as the C-glucosylxanthones mangiferin (), 2'--caffeoylmangiferin (), 2'--coumaroylmangiferin (), 2'--trans-cinnamoylmangiferin (), and the flavonoid chrysin (). The most active compound was 2'--coumaroylmangiferin () with SI > 121.9 against DENV-2 and 108.7 for HSV-1. These results indicate that mangiferin cinnamoyl esters might be potential antiviral drugs.
采用MTT法，以针对痘苗病毒西储株（VACV-WR）、人疱疹病毒1型（HSV-1）、小鼠脑心肌炎病毒（EMCV）和登革病毒2型（DENV-2）的体外试验为指导，对（紫葳科）叶、茎和果实的乙醇提取物进行了植物化学研究。所有乙醇提取物对DENV-2、HSV-1和VACV-WR均有活性，其中果实提取物对DENV-2的效果最佳（SI>38.2）。对于VACV-WR和HSV-1，测定的EC50值>200μg/mL，而未观察到EMCV对细胞病变效应的抑制作用。分离并鉴定出5种化合物，分别为C-葡萄糖基黄酮芒果苷（）、2'- - 咖啡酰芒果苷（）、2'- - 香豆酰芒果苷（）、2'- - 反式肉桂酰芒果苷（）和黄酮类化合物白杨素（）。活性最强的化合物是2'- - 香豆酰芒果苷（），对DENV-2的SI>121.9，对HSV-1的SI为108.7。这些结果表明，芒果苷肉桂酸酯可能是潜在的抗病毒药物。
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