Institut Charles Viollette (EA 7394), ISA, SFR Condorcet FR CNRS 3417, 48 bd Vauban, BP 41290, 59014, Lille, France.
Institut Charles Viollette (EA 7394), Université de Lille, SFR Condorcet FR CNRS 3417, Avenue Paul Langevin, 59655, Villeneuve d'Ascq Cedex, France.
Environ Sci Pollut Res Int. 2018 Oct;25(30):29822-29833. doi: 10.1007/s11356-017-9241-9. Epub 2017 Jun 21.
Innovation toward ecofriendly plant protection products compatible with sustainable agriculture and healthy food is today strongly encouraged. Here, we assessed the biocontrol activity of three cyclic lipopeptides from Bacillus subtilis (mycosubtilin, M; surfactin, S; fengycin, F) and two mixtures (M + S and M + S + F) on wheat against Zymoseptoria tritici, the main pathogen on this crop. Foliar application of these biomolecules at a 100-mg L concentration on the wheat cultivars Dinosor and Alixan, 2 days before fungal inoculation, provided significant reductions of disease severity. The best protection levels were recorded with the M-containing formulations (up to 82% disease reduction with M + S on Dinosor), while S and F treatments resulted in lower but significant disease reductions. In vitro and in planta investigations revealed that M-based formulations inhibit fungal growth, with half-maximal inhibitory concentrations of 1.4 mg L for both M and M + S and 4.5 mg L for M + S + F, thus revealing that the observed efficacy of these products may rely mainly on antifungal property. By contrast, S and F had no direct activity on the pathogen, hence suggesting that these lipopeptides act on wheat against Z. tritici as resistance inducers rather than as biofungicides. This study highlighted the efficacy of several lipopeptides from B. subtilis to biocontrol Z. tritici through likely distinct and biomolecule-dependent modes of action.
如今，强烈鼓励朝着与可持续农业和健康食品兼容的环保型植保产品创新。在这里，我们评估了来自枯草芽孢杆菌的三种环状脂肽（杆菌肽 M、表面活性素 S、丰原素 F）和两种混合物（M+S 和 M+S+F）对小麦纹枯病菌（Zymoseptoria tritici）的生物防治活性，纹枯病菌是这种作物的主要病原体。在小麦品种 Dinosor 和 Alixan 上，于真菌接种前 2 天叶面喷施这些生物分子，浓度为 100mg/L，可显著降低病害严重度。含 M 的制剂（在 Dinosor 上 M+S 可达到 82%的病害减少）记录了最佳的保护水平，而 S 和 F 处理则导致较低但显著的病害减少。体外和体内研究表明，M 基制剂抑制真菌生长，M 和 M+S 的半最大抑制浓度均为 1.4mg/L，M+S+F 的半最大抑制浓度为 4.5mg/L，这表明这些产品的观察到的功效可能主要依赖于抗真菌特性。相比之下，S 和 F 对病原体没有直接活性，因此表明这些脂肽作为诱导抗性物质而不是生物杀菌剂，对小麦上的 Z. tritici 起作用。本研究强调了枯草芽孢杆菌的几种脂肽通过可能不同且依赖生物分子的作用方式对 Z. tritici 进行生物防治的功效。
