Caiazzo Massimiliano, Tabata Yoji, Lutolf Matthias P
Institute of Genetics and Biophysics, "A. Buzzati-Traverso", C.N.R., Naples, 80131, Italy.
Laboratory of Stem Cell Bioengineering, Institute of Bioengineering, School of Life Sciences (SV) and School of Engineering (STI), Ecole Polytechnique Fédérale de Lausanne (EPFL), Route Cantonale, Building AI 1106, Station 15, 1015, Lausanne, Switzerland.
Methods Mol Biol. 2017;1612:65-78. doi: 10.1007/978-1-4939-7021-6_5.
Since the groundbreaking discovery of induced pluripotent stem cells (iPSCs) many research groups have attempted to improve the efficiency of the classical cell reprogramming process. Surprisingly, the contribution of the three-dimensional (3D) microenvironment to iPSC generation has been largely overlooked. Here we describe a protocol for the generation of iPSCs in defined poly(ethylene glycol) (PEG)-based hydrogels that, besides allowing higher reprogramming efficiency, are also a powerful tool to study the influence of biophysical parameters on iPSC generation.
自从诱导多能干细胞（iPSC）被开创性地发现以来，许多研究小组都试图提高经典细胞重编程过程的效率。令人惊讶的是，三维（3D）微环境对iPSC生成的贡献在很大程度上被忽视了。在这里，我们描述了一种在基于聚乙二醇（PEG）的特定水凝胶中生成iPSC的方案，该方案除了能实现更高的重编程效率外，还是研究生物物理参数对iPSC生成影响的有力工具。
