Ranftl Romana E, Calvo Fernando
Tumour Microenvironment Team, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London, SW2 6JB, UK.
Methods Mol Biol. 2017;1612:199-212. doi: 10.1007/978-1-4939-7021-6_15.
Metastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach the circulation. This is a highly dynamic process occurring in three dimensions that involves interactions between tumor, stromal cells, and the extracellular matrix. Here we describe the organotypic culture system and its utility to study breast cancer cell invasion induced by cancer-associated fibroblasts. This is a three-dimensional model that reproduces the biochemical and physiological properties of real tissue and allows for investigating the molecular and cellular mechanisms involving tumor and its microenvironment, and their contribution to cancer cell invasion . This system provides a robust, accurate, and reproducible method for measuring cancer cell invasion and represents a valuable tool to improve the mechanistic understanding of the initial steps in metastasis .
转移是癌症患者死亡的主要原因。局部肿瘤侵袭是转移扩散的关键步骤，在此过程中癌细胞从原发肿瘤脱离，穿过肿瘤周围基质并进入循环系统。这是一个在三维空间中发生的高度动态过程，涉及肿瘤、基质细胞和细胞外基质之间的相互作用。在这里，我们描述了器官型培养系统及其在研究癌症相关成纤维细胞诱导的乳腺癌细胞侵袭方面的应用。这是一个三维模型，可重现真实组织的生化和生理特性，并能够研究涉及肿瘤及其微环境的分子和细胞机制，以及它们对癌细胞侵袭的作用。该系统为测量癌细胞侵袭提供了一种强大、准确且可重复的方法，是增进对转移初始步骤机制理解的宝贵工具。
