Lowry Curley J, Moore Michael J
AxoSim Technologies, LLC, New Orleans, LA, USA.
Department of Biomedical Engineering, Lindy Boggs Center Suite 500, Tulane University, New Orleans, LA, 70118, USA.
Methods Mol Biol. 2017;1612:225-237. doi: 10.1007/978-1-4939-7021-6_17.
3D in vitro culture systems may yield physiological outcomes that more closely approximate in vivo behavior. A number of fabrication techniques and hydrogel scaffold materials are available to researchers, but often their implementation is complex and seemingly prohibitive. Herein, we describe a simplistic and adaptable dual hydrogel photolithography method utilized to engineer advanced in vitro systems for studies of neuronal development and characterization.
3D体外培养系统可能产生更接近体内行为的生理结果。研究人员可以使用多种制造技术和水凝胶支架材料，但它们的实施往往很复杂，似乎难以实现。在此，我们描述了一种简单且适应性强的双水凝胶光刻方法，用于构建先进的体外系统，以研究神经元的发育和特性。
