3D Neural Culture in Dual Hydrogel Systems.

3D in vitro culture systems may yield physiological outcomes that more closely approximate in vivo behavior. A number of fabrication techniques and hydrogel scaffold materials are available to researchers, but often their implementation is complex and seemingly prohibitive. Herein, we describe a simplistic and adaptable dual hydrogel photolithography method utilized to engineer advanced in vitro systems for studies of neuronal development and characterization.