Moeinzadeh Seyedsina, Jabbari Esmaiel
Department of Chemical Engineering, Biomimetic Materials and Tissue Engineering Laboratory, University of South Carolina, Columbia, SC, 29028, USA.
Department of Chemical Engineering, Swearingen Engineering Center, University of South Carolina, Rm 2C11, Columbia, SC, 29208, USA.
Methods Mol Biol. 2017;1612:239-252. doi: 10.1007/978-1-4939-7021-6_18.
Despite the advantages of three-dimensional (3D) hydrogels for cell culture over traditional 2D plates, their clinical application is limited by inability to recapitulate the micro-architecture of complex tissues. Micropatterning can be employed to modify the homogenous micro-architecture of hydrogels. Three techniques for cell encapsulation in 3D micropatterned gels are described. The photomask and micromold techniques are used for cell encapsulation in relatively shallow patterns like disks or short rectangles but due to the presence of PDMS mold, the resolution of micromold technique is potentially higher than the photomask. The microneedle technique is often used for cell encapsulation in relatively deep microchannels within any geometry.
尽管与传统的二维平板相比，三维（3D）水凝胶在细胞培养方面具有优势，但其临床应用受到无法重现复杂组织微观结构的限制。微图案化可用于改变水凝胶的均匀微观结构。本文描述了三种在3D微图案化凝胶中进行细胞封装的技术。光掩膜和微模具技术用于在诸如圆盘或短矩形等相对较浅的图案中进行细胞封装，但由于存在聚二甲基硅氧烷（PDMS）模具，微模具技术的分辨率可能高于光掩膜。微针技术通常用于在任何几何形状内相对较深的微通道中进行细胞封装。
