可回收微流控平台中的高通量3D肿瘤培养

High-Throughput 3D Tumor Culture in a Recyclable Microfluidic Platform.

作者信息

Liu Wenming, Wang Jinyi

机构信息

College of Science, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi, 712100, China.

出版信息

Methods Mol Biol. 2017;1612:293-301. doi: 10.1007/978-1-4939-7021-6_22.

DOI:10.1007/978-1-4939-7021-6_22

PMID:28634952

Abstract

Three-dimensional (3D) tumor culture miniaturized platforms are of importance to biomimetic model construction and pathophysiological studies. Controllable and high-throughput production of 3D tumors is desirable to make cell-based manipulation dynamic and efficient at micro-scale. Moreover, the 3D culture platform being reusable is convenient to research scholars. In this chapter, we describe a dynamically controlled 3D tumor manipulation and culture method using pneumatic microstructure-based microfluidics, which has potential applications in the fields of tissue engineering, tumor biology, and clinical medicine in a high-throughput way.

摘要

三维（3D）肿瘤培养微型平台对于仿生模型构建和病理生理研究具有重要意义。可控且高通量地生产3D肿瘤，有助于在微观尺度上使基于细胞的操作变得动态且高效。此外，3D培养平台可重复使用，这对研究学者来说很方便。在本章中，我们描述了一种使用基于气动微结构的微流体技术进行动态控制的3D肿瘤操作和培养方法，该方法在组织工程、肿瘤生物学和临床医学领域具有高通量的潜在应用。