Liu Fangzhou, Li Kaiyin, Cai Wanlun, Zhao Jing, Zou Yulan, Hua Hongxia
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
Arch Insect Biochem Physiol. 2017 Aug;95(4). doi: 10.1002/arch.21400. Epub 2017 Jun 20.
Brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is one of the most damaging pests of rice crops. BPH is a migratory insect with a delayed ovarian development in migrants classified as reproductive diapause. The molecular mechanism of reproductive diapause remains unclear, although we suspect it might be regulated by one or more nutrient signaling pathways. The target of rapamycin (TOR) pathway regulates cell growth in response to nutritional information, which raised a hypothesis that TOR mediates BPH reproductive diapause. We used a pure brachypterous strain (BS) and a predominantly macropterous strain (MS) to investigate the roles of NlTOR in BPH reproductive diapause. We found that NlTOR is expressed from the nymphal to adult stages, with a higher expression level of NlTOR in BS adults at 1, 2, and 4 days posteclosion than in MS at the same time points. Injection of dsNlTOR into BS nymphs resulted in the termination of BPH female ovary development and the retardation of nymph development. We infer that TOR signaling functions in BPH reproductive diapause by regulating the expression of NlFoxA and NlVitellogenin.
褐飞虱（BPH），褐飞虱（Nilaparvata lugens (Stål)）（半翅目：飞虱科），是水稻作物最具危害性的害虫之一。褐飞虱是一种迁飞性昆虫，迁飞个体的卵巢发育延迟，属于生殖滞育。尽管我们怀疑生殖滞育可能受一种或多种营养信号通路调控，但其分子机制仍不清楚。雷帕霉素靶蛋白（TOR）通路根据营养信息调节细胞生长，这引发了一个假设，即TOR介导褐飞虱的生殖滞育。我们使用一个纯短翅型品系（BS）和一个主要为长翅型品系（MS）来研究NlTOR在褐飞虱生殖滞育中的作用。我们发现NlTOR在若虫到成虫阶段均有表达，羽化后1天、2天和4天，BS成虫中NlTOR的表达水平高于同期的MS。向BS若虫注射dsNlTOR导致褐飞虱雌虫卵巢发育终止和若虫发育延迟。我们推断TOR信号通过调节NlFoxA和Nl卵黄原蛋白的表达在褐飞虱生殖滞育中发挥作用。
