Song Y, Huang X, Shen G H, Liu X Y, Zhang X
Department of Pathology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
Zhonghua Zhong Liu Za Zhi. 2017 Jun 23;39(6):424-428. doi: 10.3760/cma.j.issn.0253-3766.2017.06.005.
To explore the expressional differences between paired box genes 2(Pax2) and 8 (Pax8) protein in different kinds of epitheliums and tumors, and to investigate the clinicopathologic significance. Expression levels of Pax2 and Pax8 protein were detected in 75 cases of different human epithelium tissues and 255 cases of different tumors on tissue microarray by immunohistochemistry. Pax2 and Pax8 selectively expressed in different tissues. The positive rates of Pax8 protein expressed in the normal epithelium of the thyroid, urinary system and female reproductive system were 100% (2/2), 60.0% (3/5) and 76.9% (10/13), respectively. The positive rates of Pax2 expressed in the epithelium tissues of urinary system and the female reproductive system were 40.0% (2/5) and 38.5% (5/13) respectively. However, the expression of Pax2 protein was not detected in the normal thyroid epithelium. The positive rate of Pax8 protein expressing in the epithelium of reproductive system was significantly higher than that of Pax2 protein (<0.05). The tumors derived from different tissues also expressed different levels of protein Pax2 and Pax8. The positive rates of Pax8 in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were 65.2% (15/23), 66.7% (10/15) and 80.0% (4/5), respectively. The positive rates of Pax2 in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were 34.8% (8/23), 13.3% (2/15) and 20.0% (1/5), respectively. The positive rates of Pax8 protein expressed in renal cell carcinoma, thyroid carcinoma and endometrial adenocarcinoma were significantly higher than those of Pax2 protein (<0.05). The positive rates of Pax8 in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinoma were 92.9% (26/28), 81.8% (9/11) and 82.4% (14/17), respectively. The positive rates of Pax2 in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinoma were 28.6% (8/28), 9.1% (1/11) and 17.6% (3/17), respectively. The positive rates of Pax8 protein expressed in ovarian serous carcinoma, endometrial carcinoma and clear cell carcinomawere significantly higher than those of Pax2 protein (<0.05). Pax2 and Pax8 are specifically expressed in female reproductive system and uritany system. However, the positive expression of Pax8 is superior to that of Pax2. The combined expression of Pax8 and Pax2 can be used in the differential diagnosis of epithelial tumors derived from different origins.
探讨配对盒基因2（Pax2）和8（Pax8）蛋白在不同类型上皮组织及肿瘤中的表达差异，并研究其临床病理意义。采用免疫组织化学方法检测组织芯片上75例不同人体上皮组织及255例不同肿瘤中Pax2和Pax8蛋白的表达水平。Pax2和Pax8在不同组织中选择性表达。Pax8蛋白在甲状腺、泌尿系统和女性生殖系统正常上皮中的阳性率分别为100%（2/2）、60.0%（3/5）和76.9%（10/13）。Pax2在泌尿系统和女性生殖系统上皮组织中的阳性率分别为40.0%（2/5）和38.5%（5/13）。然而，在正常甲状腺上皮中未检测到Pax2蛋白的表达。Pax8蛋白在生殖系统上皮中的阳性率显著高于Pax2蛋白（<0.05）。不同组织来源的肿瘤中Pax2和Pax8蛋白的表达水平也不同。肾细胞癌、甲状腺癌和子宫内膜腺癌中Pax8的阳性率分别为65.2%（15/23）、66.7%（10/15）和80.0%（4/5）。肾细胞癌、甲状腺癌和子宫内膜腺癌中Pax2的阳性率分别为34.8%（8/23）、13.3%（2/15）和20.0%（1/5）。肾细胞癌、甲状腺癌和子宫内膜腺癌中Pax8蛋白的阳性率显著高于Pax2蛋白（<0.05）。卵巢浆液性癌、子宫内膜癌和透明细胞癌中Pax8的阳性率分别为92.9%（26/28）、81.8%（9/11）和82.4%（14/17）。卵巢浆液性癌、子宫内膜癌和透明细胞癌中Pax2的阳性率分别为28.6%（8/28）、9.1%（1/11）和17.6%（3/17）。卵巢浆液性癌、子宫内膜癌和透明细胞癌中Pax8蛋白的阳性率显著高于Pax2蛋白（<0.05）。Pax2和Pax8在女性生殖系统和泌尿系统中特异性表达。然而，Pax8的阳性表达优于Pax2。Pax8和Pax2的联合表达可用于不同来源上皮性肿瘤的鉴别诊断。
