Dalén Love, Lagerholm Vendela K, Nylander Johan A A, Barton Nick, Bochenski Zbigniew M, Tomek Teresa, Rudling David, Ericson Per G P, Irestedt Martin, Stewart John R
Department of Bioinformatics and Genetics, Swedish Museum of Natural History, Box 50007, SE-10405 Stockholm, Sweden.
Department of Zoology, Stockholm University, SE-10691 Stockholm, Sweden.
Genes (Basel). 2017 Jun 21;8(6):169. doi: 10.3390/genes8060169.
Bird remains that are difficult to identify taxonomically using morphological methods, are common in the palaeontological record. Other types of challenging avian material include artefacts and food items from endangered taxa, as well as remains from aircraft strikes. We here present a DNA-based method that enables taxonomic identification of bird remains, even from material where the DNA is heavily degraded. The method is based on the amplification and sequencing of two short variable parts of the 16S region in the mitochondrial genome. To demonstrate the applicability of this approach, we evaluated the method on a set of Holocene and Late Pleistocene postcranial bird bones from several palaeontological and archaeological sites in Europe with good success.
在古生物学记录中，很难用形态学方法进行分类鉴定的鸟类遗骸很常见。其他具有挑战性的鸟类材料类型包括濒危类群的人工制品和食物，以及飞机撞击造成的遗骸。我们在此提出一种基于DNA的方法，即使是DNA严重降解的材料，也能对鸟类遗骸进行分类鉴定。该方法基于线粒体基因组中16S区域两个短可变部分的扩增和测序。为了证明这种方法的适用性，我们在欧洲几个古生物学和考古遗址的一组全新世和晚更新世鸟类后肢骨骼上对该方法进行了评估，取得了良好的效果。
