Cheng Miffy H Y, Savoie Huguette, Bryden Francesca, Boyle Ross W
Department of Chemistry, University of Hull, Cottingham Road, Kingston-upon-Hull, East Yorkshire, HU6 7RX.
Photochem Photobiol Sci. 2017 Aug 9;16(8):1260-1267. doi: 10.1039/c7pp00091j.
Fluorescence is an essential imaging modality for labelling and visualising cells and sub-cellular structures. Multicolour labelling is especially challenging due to differences in physicochemical and photophysical behaviour of structurally unrelated fluorophores in the heterogeneous environments found in sub-cellular compartments. Herein, we report the conjugation of three azide-bearing BODIPYs with similar core structures but widely different emission wavelengths (green, red and NIR) to tyrosine residues of a model globular protein (BSA) via a common linking methodology. The resulting BODIPY-BSA conjugates have demonstrated multi-wavelength fluorescence emission for biological applications. Fluorescence imaging was performed in HeLa cells through live cell confocal microscopy imaging, with good intracellular location visualisation observed.
荧光是用于标记和可视化细胞及亚细胞结构的一种重要成像方式。由于在亚细胞区室的异质环境中，结构不相关的荧光团在物理化学和光物理行为上存在差异，多色标记尤其具有挑战性。在此，我们报告了通过一种常见的连接方法，将三种具有相似核心结构但发射波长差异很大（绿色、红色和近红外）的含叠氮基BODIPY与一种模型球状蛋白（牛血清白蛋白，BSA）的酪氨酸残基进行共轭。所得的BODIPY-BSA共轭物已证明在生物应用中具有多波长荧光发射。通过活细胞共聚焦显微镜成像在HeLa细胞中进行了荧光成像，观察到良好的细胞内定位可视化。
