一种用于检测羟基自由基的高选择性近红外荧光开启型探针及其在活细胞成像中的应用。
A Highly Selective NIR Fluorescent Turn-on Probe for Hydroxyl Radical and Its Application in Living Cell Images.
作者信息
Qu Xingyu, Song Wenting, Shen Zhen
机构信息
State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China.
Department of Chemistry and Chemical Engineering, Jinzhong University, Jinzhong, China.
出版信息
Front Chem. 2019 Aug 28;7:598. doi: 10.3389/fchem.2019.00598. eCollection 2019.
A highly selective NIR fluorescent turn-on probe for hydroxyl radical (·OH) has been built up using triphenylphosphine as a reactive-site for ·OH in an energy transfer cassette consisting of 8-2'-(thiophen-2-yl) quinoline () as a donor and 3,5-diphenylphosphinostyryl-substituted BODIPY as an acceptor, which exhibits ca. 317 nm pseudo Stokes' shift due to efficient through-bond energy transfer (up to 169%). The triphenylphosphine substituent of selectively oxidized by ·OH over the other reactive oxygen species (ROS) and the reactive nitrogen species (RNS) resulting in fluorescence enhancement in aqueous solution and in living cells.
一种用于羟基自由基（·OH）的高选择性近红外荧光开启型探针已被构建，该探针使用三苯基膦作为能量转移盒中·OH的反应位点，该能量转移盒由8-2'-（噻吩-2-基）喹啉（）作为供体和3,5-二苯基膦基苯乙烯基取代的BODIPY作为受体组成，由于有效的通过键能量转移（高达169%），该探针表现出约317 nm的伪斯托克斯位移。与其他活性氧（ROS）和活性氮（RNS）相比，三苯基膦取代基被·OH选择性氧化，导致水溶液和活细胞中的荧光增强。
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