Pulster Erin L, Main Kevan, Wetzel Dana, Murawski Steve
College of Marine Science, University of South Florida, St. Petersburg, Florida, USA.
Mote Marine Laboratory, Sarasota, Florida, USA.
Environ Toxicol Chem. 2017 Nov;36(11):3168-3176. doi: 10.1002/etc.3898. Epub 2017 Aug 7.
The 2 most abundant polycyclic aromatic hydrocarbons (PAHs) measured in Deepwater Horizon crude oil, naphthalene and phenanthrene, and their associated homologs have both been shown to be acutely toxic in fish. Although fish have a relatively high metabolic capacity for PAHs, hydroxylated PAH (OH-PAH) derivatives formed during the initial metabolic response can negatively impact the health of fish. Species-specific metabolism of naphthalene and phenanthrene was evaluated in 3 marine teleosts, red drum (Scianops ocellatus), Florida pompano (Trachinotus carolinus), and southern flounder (Paralichthys lethostigma). Fish were exposed to Deepwater Horizon crude oil by intraperitoneal injections at time 0 and 48 h, with bile sampling events at 24 and 72 h post injection. The data suggested metabolic induction in Florida pompano and red drum, whereas southern flounder may have demonstrated metabolic fatigue. By 24 h post injection, overall profiles of red drum and southern flounder were dominated by hydroxylated phenanthrene metabolites; conversely, the Florida pompano profiles were dominated by monohydroxylated naphthalenes. In addition, Florida pompano had faster overall relative biotransformation rates, suggesting their potential decreased susceptibility to adverse effects. Red drum and southern flounder had much lower relative biotransformation rates, indicating their probable susceptibility to adverse outcomes after naphthalene and phenanthrene exposures. To our knowledge, the present study is the first to investigate monohydroxylated PAHs in fish exposed to Deepwater Horizon oil. Environ Toxicol Chem 2017;36:3168-3176. © 2017 © 2017 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.
在“深水地平线”原油中检测到的两种含量最高的多环芳烃（PAH），萘和菲，以及它们相关的同系物，均已被证明对鱼类具有急性毒性。尽管鱼类对多环芳烃具有相对较高的代谢能力，但在初始代谢反应过程中形成的羟基化多环芳烃（OH-PAH）衍生物会对鱼类健康产生负面影响。在3种海洋硬骨鱼，即红鼓鱼（眼斑拟石首鱼）、佛罗里达鲳鲹和南方鲆中评估了萘和菲的种属特异性代谢。在时间0和48小时通过腹腔注射使鱼暴露于“深水地平线”原油中，并在注射后24和72小时进行胆汁采样。数据表明佛罗里达鲳鲹和红鼓鱼存在代谢诱导现象，而南方鲆可能表现出代谢疲劳。注射后24小时，红鼓鱼和南方鲆的总体代谢谱以羟基化菲代谢物为主；相反，佛罗里达鲳鲹的代谢谱以单羟基化萘为主。此外，佛罗里达鲳鲹的总体相对生物转化速率更快，表明它们对不良反应的易感性可能降低。红鼓鱼和南方鲆的相对生物转化速率低得多，表明它们在接触萘和菲后可能易受不良后果影响。据我们所知，本研究是首次调查暴露于“深水地平线”原油的鱼类中的单羟基化多环芳烃。《环境毒理学与化学》2017年；36：3168 - 3176。©2017作者。《环境毒理学与化学》由威利期刊公司代表SETAC出版。
