Amevor Felix Kwame, Cui Zhifu, Du Xiaxia, Ning Zifan, Deng Xun, Xu Dan, Wu Youhao, Cao Xueqing, Wei Shuo, Shu Gang, Han Xue, Tian Yaofu, Li Diyan, Wang Yan, Zhang Yao, Du Xiaohui, Zhu Qing, Zhao Xiaoling
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
Department of Basic Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Front Physiol. 2022 Apr 11;13:873551. doi: 10.3389/fphys.2022.873551. eCollection 2022.
The current study aims to investigate the effects of the synergy between quercetin and vitamin E in aged hen's diet on hatchability and antioxidant levels of the embryo and newly hatched chicks from prolonged storage eggs. A total of 400 breeder laying hens of 65 weeks of age were selected and randomly divided into 4 groups. Birds were fed a basal diet alone (Control), and basal diets supplemented with quercetin (Q) (0.4 g/kg) and vitamin E (VE) (0.2 g/kg) alone and their combination (0.4 g/kg Q + 0.2 g/kg VE) for 14 weeks, respectively, to determine their effects on yolk antioxidant status, fertility, embryonic mortality, hatchability, antioxidant status of embryonic tissues, as well as the antioxidant status of the newly hatched chicks. The results showed that the hen's dietary Q + VE increased the yolk weight, as well as increased the antioxidant status of the egg yolk ( < 0.05). Compared with the control group, the supplementation of Q + VE significantly increased the hatchability of set-fertile eggs and decreased early embryonic mortality in eggs stored for 7 and 14 days, respectively ( < 0.05), and also improved the antioxidant capacity of the embryos obtained from eggs stored for 14 days (before incubation) ( < 0.05). Moreover, Q + VE increased the levels of SOD, GSH-Px, T-AOC, T-SOD, and CAT in the liver, heart, and pectoral muscle of the embryo, 1-day-old and 14-day-old chicks ( < 0.05), as well as upregulated the antioxidant related genes (GPx-1, GPx-2, GPx-4, DIO-1, and SOD-1) in the liver of the embryo, 1-day-old and 14-day-old chicks hatched from 14-days storage eggs ( < 0.05). Meanwhile, the MDA levels were decreased by the Q + VE in the embryo and post-hatched chicks ( < 0.05). In conclusion, these findings suggested that maternal dietary Q + VE exerts beneficial synergistic effects on the antioxidant capacity of the egg yolk, embryo, and chicks during prolong egg storage, therefore, Q + VE could be used as a dietary measure to enhance hatchability and chick quality in poultry production.
本研究旨在探讨槲皮素与维生素E协同作用于老龄母鸡日粮时，对长期储存鸡蛋所孵化胚胎及新孵出雏鸡的孵化率和抗氧化水平的影响。选取400只65周龄的种母鸡，随机分为4组。分别给鸡单独饲喂基础日粮（对照组），以及在基础日粮中单独添加槲皮素（Q）（0.4 g/kg）、维生素E（VE）（0.2 g/kg）及其组合（0.4 g/kg Q + 0.2 g/kg VE），持续14周，以确定它们对蛋黄抗氧化状态、受精率、胚胎死亡率、孵化率、胚胎组织抗氧化状态以及新孵出雏鸡抗氧化状态的影响。结果表明，母鸡日粮中添加Q + VE可增加蛋黄重量，并提高蛋黄的抗氧化状态（P<0.05）。与对照组相比，添加Q + VE显著提高了储存7天和14天的种蛋的孵化率，并分别降低了这些蛋的早期胚胎死亡率（P<0.05），还提高了从储存14天的鸡蛋（孵化前）获得的胚胎的抗氧化能力（P<0.05）。此外，Q + VE提高了胚胎、1日龄和14日龄雏鸡肝脏、心脏和胸肌中SOD、GSH-Px、T-AOC、T-SOD和CAT的水平（P<0.05），并上调了从储存14天的鸡蛋孵化出的胚胎、1日龄和14日龄雏鸡肝脏中抗氧化相关基因（GPx-1、GPx-2、GPx-4、DIO-1和SOD-1）的表达（P<0.05）。同时，Q + VE降低了胚胎和出壳后雏鸡的MDA水平（P<0.05）。总之，这些研究结果表明，母鸡日粮中添加Q + VE在延长鸡蛋储存期间对蛋黄、胚胎和雏鸡的抗氧化能力具有有益的协同作用，因此，Q + VE可作为一种日粮措施来提高家禽生产中的孵化率和雏鸡质量。
