大鼠眼角膜碱烧伤的综合建模
Comprehensive Modeling of Corneal Alkali Injury in the Rat Eye.
作者信息
Choi Hosoon, Phillips Casie, Oh Joo Youn, Stock Eileen M, Kim Dong-Ki, Won Jae-Kyung, Fulcher Samuel
机构信息
a Department of Basic Research , Central Texas Veterans Research Foundation , Temple , TX , USA.
b Department of Ophthalmology , Seoul National University Hospital, Daehak-ro, Jongno-gu , Seoul , Republic of Korea.
出版信息
Curr Eye Res. 2017 Oct;42(10):1348-1357. doi: 10.1080/02713683.2017.1317817. Epub 2017 Jun 21.
PURPOSE
To characterize the molecular, clinical, and histopathological profiles in the rat cornea after alkali injury over a 21-day period.
METHODS
Alkali injury was induced in one eye of male Lewis rats. Corneal opacity and corneal neovascularization were assessed daily. Real-time qRT-PCR analysis and immunohistochemical staining were conducted to examine inflammation, neovascularization, and fibrosis.
RESULTS
We found that within 2 hours of chemical exposure, corneal opacification rapidly developed with an acute increase in various cytokine expressions, while several cytokines demonstrated a secondary peak by day 7. Early neutrophil infiltration peaked at day 1 post-injury while macrophage infiltration peaked at day 7. Throughout the time course of the study, corneal opacity persisted and neovascularization, lymphangiogenesis, and fibrosis progressed.
CONCLUSIONS
This study highlights the molecular, clinical, and histopathological changes throughout the progression of alkali injury in the rat cornea. These profiles will assist in the development of new strategies and therapies for ocular alkali injury.
目的
描述大鼠角膜碱烧伤后21天内的分子、临床和组织病理学特征。
方法
对雄性Lewis大鼠的一只眼睛进行碱烧伤。每天评估角膜混浊和角膜新生血管形成情况。采用实时定量逆转录聚合酶链反应（qRT-PCR）分析和免疫组织化学染色来检测炎症、新生血管形成和纤维化情况。
结果
我们发现，化学物质暴露后2小时内，角膜迅速发生混浊，各种细胞因子表达急剧增加，而几种细胞因子在第7天出现第二个峰值。早期中性粒细胞浸润在损伤后第1天达到峰值，而巨噬细胞浸润在第7天达到峰值。在整个研究过程中，角膜混浊持续存在，新生血管形成、淋巴管生成和纤维化不断进展。
结论
本研究突出了大鼠角膜碱烧伤进展过程中的分子、临床和组织病理学变化。这些特征将有助于开发针对眼部碱烧伤的新策略和治疗方法。
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