在蜡螟（Galleria mellonella）体内的传代是否会像在小鼠模型中那样激活巴西副球孢子菌的毒力因子？

Can passage in Galleria mellonella activate virulence factors of Paracoccidioides brasiliensis as in the murine model?

作者信息

Scorzoni Liliana, de Paula E Silva Ana Carolina Alves, de Oliveira Haroldo Cesar, Marcos Caroline Maria, Singulani Junya de Lacorte, Fusco-Almeida Ana Marisa, Mendes-Giannini Maria José Soares

机构信息

Laboratório de Micologia Clínica, Departamento de Análises Clínicas, Universidade Estadual Paulista (UNESP), Faculdade de Ciências Farmacêuticas, Araraquara, Brasil.

出版信息

Med Mycol. 2018 Apr 1;56(3):374-377. doi: 10.1093/mmy/myx045.

DOI:10.1093/mmy/myx045

PMID:28637229

Abstract

Paracoccidioidomycosis (PCM) is a fungal disease restricted to Latin countries, and its etiologic agents derive from the Paracoccidioides genus. Attenuation or loss of virulence in Paracoccidioides spp. following successive subculturing has been described. However, virulence can be recovered by passage in mammalian host. In this study, the recovery of adhesion of P. brasiliensis through passage in mice was compared to that in the insect Galleria mellonella. Analysis of in vitro fungal-host cell interaction, gene expression of adhesins, and analysis of the survival curves revealed that Galleria mellonella is useful for the reactivation of P. brasiliensis adhesion.

摘要

副球孢子菌病（PCM）是一种局限于拉丁国家的真菌病，其病原体源自副球孢子菌属。已报道副球孢子菌属在连续传代培养后毒力减弱或丧失。然而，通过在哺乳动物宿主体内传代可恢复毒力。在本研究中，将巴西副球孢子菌在小鼠体内传代后黏附力的恢复情况与在昆虫大蜡螟体内的情况进行了比较。对体外真菌与宿主细胞相互作用、黏附素基因表达的分析以及生存曲线分析表明，大蜡螟有助于巴西副球孢子菌黏附力的恢复。