Casaglia O, Dore E, Frontali C, Zenobi P, Walliker D
Mol Biochem Parasitol. 1985 Jun;16(1):35-42. doi: 10.1016/0166-6851(85)90047-7.
Previous results, relating mosquito infectivity to percentage of repetitive DNA in the genome of Plasmodia, are re-examined in the light of the finding that a parasite line used in the previous studies and classified as Plasmodium berghei NK65, was a mixed infection, where the major component appeared to be Plasmodium yoelii. This conclusion was reached through cloning and isoenzyme typing of different clones. Isoenzyme typing alone is not sufficiently sensitive to reveal contamination amounting to less than 20% in a mixture. Attention is drawn to the risk inherent in work with uncloned lines, where the proportions of species or sub-species present may vary according to line history and gametocyte viability.
鉴于之前研究中使用的并被归类为伯氏疟原虫NK65的寄生虫株是一种混合感染，其中主要成分似乎是约氏疟原虫，因此根据这一发现，重新审视了之前关于蚊子感染性与疟原虫基因组中重复DNA百分比之间关系的结果。这一结论是通过对不同克隆进行克隆和同工酶分型得出的。仅同工酶分型的灵敏度不足以揭示混合物中低于20%的污染情况。需要注意的是，使用未克隆的虫株进行研究存在固有风险，其中存在的物种或亚种比例可能因虫株历史和配子体活力而有所不同。
