Institute of Microbiology and College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
Sci Rep. 2017 Jun 21;7(1):3932. doi: 10.1038/s41598-017-04285-5.
Because of ubiquity of thioesters, thioesterases play a critical role in metabolism, membrane biosynthesis, signal transduction, and gene regulation. In many bacteria, YbgC is such an enzyme, whose coding gene mostly resides in the tol-pal cluster. Although all other proteins encoded in the tol-pal cluster are clearly involved in maintaining cell envelope integrity and cell division, little is known about the physiological role of YbgC. In this study, we identify in Shewanella oneidensis, a γ-proteobacterium used as a research model for environmental microbes, YbgC as a motility regulator. The loss of YbgC results in enhanced motility, which is likely due to the increased rotation rate of the flagellum. The regulatory function of YbgC requires its thioesterase activity but could not be replaced by YbgC homologues of other bacteria. We further show that the regulation of YbgC is mediated by the second message c-di-GMP.
由于硫酯的普遍存在，硫酯酶在代谢、膜生物合成、信号转导和基因调控中起着关键作用。在许多细菌中，YbgC 就是这样一种酶，其编码基因大多位于 tol-pal 簇中。尽管 tol-pal 簇中编码的所有其他蛋白质显然都参与维持细胞包膜的完整性和细胞分裂，但关于 YbgC 的生理作用知之甚少。在这项研究中，我们在 Shewanella oneidensis 中鉴定出 YbgC，它是一种用于环境微生物研究的γ-变形菌。YbgC 作为一种运动调节剂。YbgC 的缺失导致运动性增强，这可能是由于鞭毛的旋转速度增加。YbgC 的调节功能需要其硫酯酶活性，但不能被其他细菌的 YbgC 同源物取代。我们进一步表明，YbgC 的调节是由第二信使 c-di-GMP 介导的。
