Wei Xi, Li Yi-Bo, Li Ying, Lin Ben-Cheng, Shen Xiao-Min, Cui Ran-Liang, Gu Ya-Jun, Gao Ming, Li Yue-Guo, Zhang Sheng
Department of Diagnostic and Therapeutic Ultrasonography, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.
Department of general surgery, The first affiliated hospital of DaLian medical university.
J Cancer. 2017 Jun 1;8(8):1492-1497. doi: 10.7150/jca.18615. eCollection 2017.
To investigate the functional role of serum Human apurinic/apyrimidinic endonuclease 1 (APE1) in prediction of lymph node metastasis in gastric cancer patients. Serum samples were pre-operational collected from 86 patients with gastric cancer from Tianjin Medical University Cancer Institute and Hospital from March 2016 to August 2016. The serum of APE1 was measured by ELISA development kit and other CA242, CA724, CA199 and CEA levels by electrochemiluminescence assay. The total of 86 patients with gastric cancer was classified into two groups (lymph node positive and negative groups). Using ELISA assay, we found out that the concentration of serum APE1 was higher in lymph node positive group than that of lymph node negative group. The receiver operating characteristic (ROC) curve was performed to analyze, indicating that area under the ROC curve of serum APE1 were better than those of each regular markers (CEA+CA199+CA242+CA724) or combination of these markers. Additionally, the APE1 overexpression was uncovered in tissue of gastric cancer patients with lymph nodes metastases, which is correlation with results of serum APE1. Serum APE1 was identified as a valuable marker for prediction of lymph node metastases in patients with gastric cancer.
探讨血清人脱嘌呤/脱嘧啶内切酶1（APE1）在预测胃癌患者淋巴结转移中的功能作用。2016年3月至2016年8月期间，从天津医科大学肿瘤医院收集了86例胃癌患者的术前血清样本。采用ELISA检测试剂盒检测血清APE1水平，采用电化学发光法检测其他CA242、CA724、CA199和CEA水平。86例胃癌患者分为两组（淋巴结阳性组和阴性组）。通过ELISA检测，我们发现淋巴结阳性组血清APE1浓度高于淋巴结阴性组。绘制受试者工作特征（ROC）曲线进行分析，结果表明血清APE1的ROC曲线下面积优于各常规标志物（CEA+CA199+CA242+CA724）或这些标志物的组合。此外，在有淋巴结转移的胃癌患者组织中发现了APE1的过表达，这与血清APE1的结果相关。血清APE1被确定为预测胃癌患者淋巴结转移的有价值标志物。
