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表没食子儿茶素诱导人宫颈癌细胞系凋亡的研究

APOPTOSIS INDUCTION OF EPIFRIEDELINOL ON HUMAN CERVICAL CANCER CELL LINE.

作者信息

Yang Jie, Fa Jing, Li Bingxing

机构信息

Women's Health Center, Women and Children's Hospital of LinYi, Linyi, Shandong, China, 276000.

Department of Gynaecology, Linyi Cancer Hospital, Linyi, Shandong, China, 276000.

出版信息

Afr J Tradit Complement Altern Med. 2017 Jun 5;14(4):80-86. doi: 10.21010/ajtcam.v14i4.10. eCollection 2017.

DOI:10.21010/ajtcam.v14i4.10
PMID:28638870
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5471486/
Abstract

BACKGROUND

Present investigation evaluates the antitumor activity of epifriedelinol for the management of cervical cancer by inducing process of apoptosis.

METHODS

Human Cervical Cancer Cell Line, C33A and HeLa were selected for study and treated with epifriedelinol at a concentration of (50-1000 µg/ml). Cytotoxicity of epifriedelinol was estimated by MTT assay and induction of apoptosis was assessed by estimating the activity of caspase 3, 8 and 9 enzyme, apoptosis assay and translocation of cytochrome c. Moreover an expression of several proteins that plays role in the apoptosis process was estimated by western blot method.

RESULTS

Result of the study suggested that treatment with epifriedelinol significantly decrease the viability count of cancerous cell in a dose perndent manner and also enhances the formation of oligonucleosome in both the cell lines. However activity of caspase enzymes and translocation of cytochrome c were enhanced after treatment with epifriedelinol. It was also observed that epifriedelinol treatment alters the ratio of pro-apoptotic to anti-apoptotic proteins and enhances the expressions of inhibitor of apoptosis proteins (IAP).

CONCLUSION

Result of our study proves the anticancer activity of epifriedelinol in cervical cancer by inducing apoptosis as treatment with it enhances the production of oligonucleosomes, translocation of cytochrome c and activity caspase enzymes.

摘要

背景

本研究通过诱导凋亡过程评估表木栓醇对宫颈癌的抗肿瘤活性。

方法

选择人宫颈癌细胞系C33A和HeLa进行研究，并用浓度为（50 - 1000μg/ml）的表木栓醇处理。通过MTT法评估表木栓醇的细胞毒性，并通过评估半胱天冬酶3、8和9的活性、凋亡检测以及细胞色素c的转位来评估凋亡诱导情况。此外，通过蛋白质印迹法评估在凋亡过程中起作用的几种蛋白质的表达。

结果

研究结果表明，表木栓醇处理以剂量依赖性方式显著降低癌细胞的活力计数，并增强两种细胞系中寡核小体的形成。然而，用表木栓醇处理后，半胱天冬酶的活性和细胞色素c的转位增强。还观察到表木栓醇处理改变了促凋亡蛋白与抗凋亡蛋白的比例，并增强了凋亡抑制蛋白（IAP）的表达。

结论

我们的研究结果证明了表木栓醇在宫颈癌中的抗癌活性，因为用它处理可增强寡核小体的产生、细胞色素c的转位和半胱天冬酶的活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/b4c81c531272/AJTCAM-14-80-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/5ab6df7645cf/AJTCAM-14-80-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/cd344b4d6cf5/AJTCAM-14-80-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/0e11bcdd1f5e/AJTCAM-14-80-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/2eb08762b7bf/AJTCAM-14-80-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/bcad8bcbd8ce/AJTCAM-14-80-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/b4c81c531272/AJTCAM-14-80-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/5ab6df7645cf/AJTCAM-14-80-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/cd344b4d6cf5/AJTCAM-14-80-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/0e11bcdd1f5e/AJTCAM-14-80-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/2eb08762b7bf/AJTCAM-14-80-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/bcad8bcbd8ce/AJTCAM-14-80-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ddfa/5471486/b4c81c531272/AJTCAM-14-80-g006.jpg

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