Romay Gustavo, Chirinos Dorys T, Geraud-Pouey Francis, Gillis Annika, Mahillon Jacques, Desbiez Cécile, Bragard Claude
Earth and Life Institute, Applied Microbiology - Phytopathology, Université catholique de Louvain, Croix du Sud 2 Bte L07.05.03, 1348, Louvain-la-Neuve, Belgium.
Unidad Técnica Fitosanitaria, La Universidad del Zulia (LUZ), Maracaibo, 4005, Estado Zulia, Venezuela.
Virus Genes. 2017 Dec;53(6):939-942. doi: 10.1007/s11262-017-1478-3. Epub 2017 Jun 20.
Tomato mild yellow leaf curl Aragua virus (ToMYLCV) is a begomovirus first reported infecting tomato (Solanum lycopersicum) and milkweed (Euphorbia heterophylla) in Venezuela. In this study, a ToMYLCV isolate (Zulia-219) was completely sequenced and its host range was evaluated. The DNA-A and DNA-B components of isolate Zulia-219 showed 93 and 85% nucleotide sequence identity with the respective counterparts of the ToMYLCV type strain. According to current demarcation criteria for begomovirus species, Zulia-219 is a new strain of ToMYLCV. Interestingly, tomato plants inoculated with ToMYLCV Zulia-219 displayed severe symptoms, including severe chlorotic leaf curling, in contrast to mild symptoms associated with the type strain of this begomovirus. These results indicate potential risks associated with this new ToMYLCV strain for tomato production in Venezuela.
番茄温和黄化曲叶阿拉瓜病毒（ToMYLCV）是一种双生病毒，首次报道于委内瑞拉，可感染番茄（Solanum lycopersicum）和泽漆（Euphorbia heterophylla）。在本研究中，对一株ToMYLCV分离株（Zulia-219）进行了全序列测定，并评估了其寄主范围。分离株Zulia-219的DNA-A和DNA-B组分与ToMYLCV模式株的相应组分分别具有93%和85%的核苷酸序列同一性。根据目前双生病毒属物种的划分标准，Zulia-219是ToMYLCV的一个新株系。有趣的是，接种ToMYLCV Zulia-219的番茄植株表现出严重症状，包括严重的褪绿卷叶，而该双生病毒模式株系引起的症状较轻。这些结果表明，这种新的ToMYLCV株系对委内瑞拉的番茄生产存在潜在风险。
