Elde R, Johansson O, Hokfelt T
Adv Exp Med Biol. 1985;188:167-81. doi: 10.1007/978-1-4615-7886-4_10.
Immunohistochemical studies with antisera to somatostatin have, in many instances, led the way to our present understanding of the peptidergic nervous system. Somatostatin was among the first of the hypophysiotropic hormones shown to be contained in diverse neuronal circuits outside of the hypothalamus. For example, somatostatin is found within neurons ranging in location from the cerebral cortex to primary sensory neurons to enteric neurons within the gut wall. Somatostatin was also the first neuropeptide demonstrated to coexist within vertebrate neurons that also produce a classical neurotransmitter. Since this initial demonstration in sympathetic ganglionic neurons, somatostatin and numerous other neuropeptides have been demonstrated to coexist with a variety of classical neurotransmitters. The "rules" for coexistence are not clear, since somatostatin coexist in some instances with norepinephrine, in other cases with GABA, and probably with other classical transmitters as well. In some neurons, somatostatin also coexists with certain other neuropeptides. Finally, the specificity of immunohistochemical localizations of somatostatin has now been confirmed by virtue of the co-staining of somatostatin neurons with antisera to other portions of the biosynthetic precursor to somatostatin.
使用抗生长抑素抗血清进行的免疫组织化学研究，在许多情况下，引领了我们目前对肽能神经系统的理解。生长抑素是最早被证明存在于下丘脑以外各种神经回路中的促垂体激素之一。例如，在从大脑皮层到初级感觉神经元再到肠壁内的肠神经元等不同位置的神经元中都能发现生长抑素。生长抑素也是第一个被证明与也产生经典神经递质的脊椎动物神经元共存的神经肽。自从在交感神经节神经元中首次证明这种共存以来，生长抑素和许多其他神经肽已被证明与多种经典神经递质共存。共存的“规则”尚不清楚，因为生长抑素在某些情况下与去甲肾上腺素共存，在其他情况下与γ-氨基丁酸共存，可能还与其他经典递质共存。在一些神经元中，生长抑素还与某些其他神经肽共存。最后，生长抑素免疫组织化学定位的特异性现已通过生长抑素神经元与生长抑素生物合成前体其他部分的抗血清共染色得到证实。
