Šefčíková Z, Raček L
Institute of Animal Physiology, Slovak Academy of Sciences , Košice, Slovak Republic.
Physiol Int. 2016 Jun 1;103(2):183-190. doi: 10.1556/036.103.2016.2.5.
Purpose The objective of this study was to evaluate whether pectin feeding would affect the small intestinal function and whether these changes would lead to obesity prevention in rats fed with high-fat diet. Three groups of weaned male rats (ad lib. fed; rats fed with diet containing 15% w/w of citrus pectin; restrictedly pair-fed rats) were fed with either a standard diet (9.5% fat) or a high-fat diet (30% fat) for 10 days. Results Our results revealed that pectin feeding led to significant decreases in body weight, energy intake and fat pad weight in rats fed with the standard as well as high-fat diet. Moreover, compared to the restrictedly pair-fed rats, in both groups of rats fed with the diet containing pectin, significant decrease in duodenal alkaline phosphatase (AP) activity was observed in histochemically stained cryostat sections. In contrast, despite their lower energy intake, restrictedly pair-fed rats showed similar fat pad deposition accompanied by unchanged values of AP activity in comparison to the controls. Conclusions Our findings indicate that daily pectin consumption could be beneficial in suppressing body weight gain and reducing probability of obesity risk in rats fed with a high-fat diet.
目的 本研究的目的是评估喂食果胶是否会影响小肠功能，以及这些变化是否会导致高脂饮食喂养的大鼠预防肥胖。将三组断奶雄性大鼠（自由采食；喂食含15% w/w柑橘果胶饮食的大鼠；限食配对喂养大鼠）分别喂食标准饮食（9.5%脂肪）或高脂饮食（30%脂肪）10天。结果 我们的结果显示，喂食果胶导致喂食标准饮食和高脂饮食的大鼠体重、能量摄入和脂肪垫重量显著降低。此外，与限食配对喂养大鼠相比，在两组喂食含果胶饮食的大鼠中，在组织化学染色的低温恒温切片中观察到十二指肠碱性磷酸酶（AP）活性显著降低。相比之下，尽管限食配对喂养大鼠的能量摄入较低，但与对照组相比，其脂肪垫沉积相似，AP活性值未改变。结论 我们的研究结果表明，每日食用果胶可能有利于抑制高脂饮食喂养大鼠的体重增加并降低肥胖风险概率。
