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对南马托格罗索州发现的埃及伊蚊幼虫(林奈,1762年)(蚊科)进行植物提取物和馏分的筛选。

Screening of plant extracts and fractions on Aedes aegypti larvae found in the state of Mato Grosso do Sul (linnaeus, 1762) (culicidae).

作者信息

Porto Karla R DE Andrade, Motti Priscilla R, Yano Mami, Roel Antonia R, Cardoso Claudia A L, Matias Rosemary

机构信息

Universidade Católica Dom Bosco/UCDB, Av. Tamandaré, 6000, 79117-900 Campo Grande, MS, Brazil.

Faculdade Estácio de Sá de Campo Grande, Mato Grosso do Sul, R.Venâncio Borges do Nascimento, 377, 79050-700 Campo Grande, MS, Brazil.

出版信息

An Acad Bras Cienc. 2017 Apr-Jun;89(2):895-906. doi: 10.1590/0001-3765201720150017.

DOI:10.1590/0001-3765201720150017
PMID:28640344
Abstract

The constant use of chemical insecticides for Aedes aegypti control has caused resistance in the mosquito populations. Thus, the objective of this study was to analyze the larvicidal potential of extracts and fractions of plants on A. aegypti larvae. The analysis included sixty one extracts and twenty five fractions of fifty botanical species at concentrations of 0.25; 0.12; 0.06 to 0.03 mg mL-1; 4 replications and one negative control of dechlorinate water and 1% DMSO; and a positive control with rotenone. The toxicity index in descending order with LC50 for the most active of the extracts selected were ethanol extract of Ormosea arborea (0.111 mg mL-1) seeds and ethanol extracts of leaves such as Piper hispidum (0.169 mg mL-1), Solanum variabile (0.188 mg mL-1), O. arborea (0.238 mg mL-1), Turnera umifolia (0.242 mg mL-1) and Piper hispidum (0.567 mg mL-1). For plant fractions, the most active were chloroform (0.192 mg mL-1) and hexane (0.342 mg mL-1) P. aduncum leaves, hexane fraction (0.415 mg mL-1) and methanol extract (0.625 mg mL-1) of Spermacocea latifolia leaves. Regarding the extract of T. umifolia single species, there is no bibliographic report on their degree of efficiency as an insecticide.

摘要

持续使用化学杀虫剂来控制埃及伊蚊已导致蚊虫种群产生抗性。因此,本研究的目的是分析植物提取物和馏分对埃及伊蚊幼虫的杀幼虫潜力。分析包括来自50种植物的61种提取物和25种馏分,浓度分别为0.25、0.12、0.06至0.03mg/mL;4次重复,一个用去氯水和1%DMSO作为阴性对照;以及用鱼藤酮作为阳性对照。对于所选最具活性的提取物,按LC50降序排列的毒性指数依次为:奥氏红豆树种子乙醇提取物(0.111mg/mL)、毛胡椒叶乙醇提取物(0.169mg/mL)、变异茄叶乙醇提取物(0.188mg/mL)、奥氏红豆树叶乙醇提取物(0.238mg/mL)、单花金午时花乙醇提取物(0.242mg/mL)和毛胡椒叶乙醇提取物(0.567mg/mL)。对于植物馏分,最具活性的是钩腺大戟叶的氯仿馏分(0.192mg/mL)和己烷馏分(0.342mg/mL)、阔叶丰花草叶的己烷馏分(0.415mg/mL)和甲醇提取物(0.625mg/mL)。关于单花金午时花单一物种的提取物,尚无关于其作为杀虫剂的效率程度的文献报道。

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