海蟾蜍角膜上皮细胞对氨基酸的转运

Amino acid transport by corneal epithelial cells from the toad, Bufo marinus.

作者信息

Cooperstein D F

出版信息

Comp Biochem Physiol A Comp Physiol. 1985;81(2):427-30. doi: 10.1016/0300-9629(85)90159-8.

Abstract

alpha-Aminoisobutyric acid is actively accumulated by the epithelium of the cornea of the toad, Bufo marinus, resulting in a tissue to medium ratio of 4 to 1 after 40 min of incubation. The accumulation of alpha-aminoisobutyric acid is ouabain-sensitive and dependent upon the presence of extracellular sodium. Transport is inhibited by carbon monoxide, 6-aminonicotinamide, arsenite and n-heptyl-3-hydroxy-quinoline-n-oxide and stimulated by diamide.

摘要

α-氨基异丁酸被海蟾蜍（Bufo marinus）角膜上皮细胞主动积累，孵育40分钟后组织与培养基的比例达到4比1。α-氨基异丁酸的积累对哇巴因敏感，且依赖于细胞外钠的存在。一氧化碳、6-氨基烟酰胺、亚砷酸盐和正庚基-3-羟基喹啉-N-氧化物可抑制其转运，而二酰胺可刺激其转运。