牛赖氨酰氧化酶的免疫学特性

Immunological characterization of bovine lysyl oxidase.

作者信息

Burbelo P D, Kagan H M, Chichester C O

出版信息

Comp Biochem Physiol B. 1985;81(4):845-9. doi: 10.1016/0305-0491(85)90077-x.

Abstract

Antibodies to homogeneously purified bovine aortic lysyl oxidase were prepared in chickens. The chicken anti-lysyl oxidase antiserum effectively inhibited bovine aortic lysyl oxidase activity. Non-immune antiserum from chickens, goats and humans was found to enhance bovine aortic lysyl oxidase activity, while non-immune rabbit serum inhibited enzyme activity. A competitive ELISA was developed to monitor immunoreactive lysyl oxidase during purification. Chromatography of bovine lysyl oxidase on Sephacryl S-200, the final step in purification, revealed two peaks of immunoreactive lysyl oxidase. The large molecular weight peak appears to contain inactive multimeric forms of the enzyme.

摘要

在鸡体内制备了针对经均匀纯化的牛主动脉赖氨酰氧化酶的抗体。鸡抗赖氨酰氧化酶抗血清有效抑制了牛主动脉赖氨酰氧化酶的活性。发现来自鸡、山羊和人类的非免疫抗血清可增强牛主动脉赖氨酰氧化酶的活性，而兔非免疫血清则抑制该酶的活性。开发了一种竞争性酶联免疫吸附测定法来监测纯化过程中具有免疫反应性的赖氨酰氧化酶。牛赖氨酰氧化酶在Sephacryl S - 200上进行色谱分析（纯化的最后一步），显示出两个具有免疫反应性的赖氨酰氧化酶峰。高分子量峰似乎包含该酶的无活性多聚体形式。