赖氨酸氧化酶的抑制作用的存在以及多种形式的存在。

The existence of inhibited lysyl oxidase and the presence of multiple forms.

作者信息

Shieh J J, Sasaki T, Minamiura N, Yasunobu K T

出版信息

Adv Exp Med Biol. 1977;79:509-29. doi: 10.1007/978-1-4684-9093-0_43.

Abstract

Considerable difficulty in puriying tissue lysyl oxidase has been previously encountered and the cause of this difficulty has now been ascertained. For these studies, we have used blood plasma lysyl oxidase as a model system. Blood plasma has been shown to contain considerable lysyl oxidase activity. The enzyme exists in a partially or completely inhibited state. Homogeneous preparations of the enzyme from the blood have been obtained by two different methods. Method A yields one form of lysyl oxidase while Method B yields three forms of the enzyme. The methods for the purification of the enzyme and some properties of the enzyme and preliminary data on the lysyl oxidase inhibitor will be discussed.

摘要

先前在纯化组织赖氨酰氧化酶时遇到了相当大的困难，现在已经查明了造成这种困难的原因。在这些研究中，我们使用血浆赖氨酰氧化酶作为模型系统。已证明血浆中含有相当数量的赖氨酰氧化酶活性。该酶以部分或完全抑制的状态存在。通过两种不同的方法获得了血液中该酶的纯制剂。方法A产生一种形式的赖氨酰氧化酶，而方法B产生三种形式的该酶。将讨论该酶的纯化方法、酶的一些性质以及赖氨酰氧化酶抑制剂的初步数据。