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IbOr通过稳定甘薯中的IbPsbP在热胁迫下调节光合作用。

IbOr Regulates Photosynthesis under Heat Stress by Stabilizing IbPsbP in Sweetpotato.

作者信息

Kang Le, Kim Ho S, Kwon Young S, Ke Qingbo, Ji Chang Y, Park Sung-Chul, Lee Haeng-Soon, Deng Xiping, Kwak Sang-Soo

机构信息

Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and BiotechnologyDaejeon, South Korea.

Department of Green Chemistry and Environmental Biotechnology, Korea University of Science and TechnologyDaejeon, South Korea.

出版信息

Front Plant Sci. 2017 Jun 8;8:989. doi: 10.3389/fpls.2017.00989. eCollection 2017.

DOI:10.3389/fpls.2017.00989
PMID:28642783
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5462972/
Abstract

The Orange (Or) protein regulates carotenoid biosynthesis and environmental stress in plants. Previously, we reported that overexpression of the sweetpotato [ (L.) Lam] gene () in transgenic (referred to as -OX/At) increased the efficiency of photosystem II (PSII) and chlorophyll content after heat shock. However, little is known about the role of IbOr in PSII-mediated protection against abiotic stress. In this study, comparative proteomics revealed that expression of PsbP (an extrinsic subunit of PSII) is up-regulated in heat-treated -OX/At plants. We then identified and functionally characterized the - gene () from sweetpotato. IbPsbP is predominantly localized in chloroplast, and its transcripts are tissue-specifically expressed and up-regulated in response to abiotic stress. In addition, IbOr interacts with IbPsbP and protects it from heat-induced denaturation, consistent with the observation that transgenic sweetpotato overexpressing maintained higher PSII efficiency and chlorophyll content upon exposure to heat stress. These results indicate that IbOr can protect plants from environmental stress not only by controlling carotenoid biosynthesis but also by directly stabilizing PSII.

摘要

橙色（Or）蛋白调控植物中的类胡萝卜素生物合成和环境胁迫。此前，我们报道过在转基因拟南芥（称为IbOr - OX/At）中过量表达甘薯[Ipomoea batatas (L.) Lam]基因（IbOr）可提高热激后光系统II（PSII）的效率和叶绿素含量。然而，关于IbOr在PSII介导的非生物胁迫保护中的作用知之甚少。在本研究中，比较蛋白质组学显示，热处理的IbOr - OX/At植物中PSII的外在亚基PsbP的表达上调。然后，我们从甘薯中鉴定并对IbPsbP基因进行了功能表征。IbPsbP主要定位于叶绿体，其转录本在组织中特异性表达，并响应非生物胁迫而上调。此外，IbOr与IbPsbP相互作用并保护其免受热诱导的变性，这与过表达IbOr的转基因甘薯在热胁迫下保持较高PSII效率和叶绿素含量的观察结果一致。这些结果表明，IbOr不仅可以通过控制类胡萝卜素生物合成，还可以通过直接稳定PSII来保护植物免受环境胁迫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/e992db920987/fpls-08-00989-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/5f2fd4d62dc1/fpls-08-00989-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/250107b1537b/fpls-08-00989-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/a2413f776034/fpls-08-00989-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/6f52ed7b7329/fpls-08-00989-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/f561df627863/fpls-08-00989-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/6cbd29ff2138/fpls-08-00989-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/1a183bb2c0dd/fpls-08-00989-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/e992db920987/fpls-08-00989-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/5f2fd4d62dc1/fpls-08-00989-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/250107b1537b/fpls-08-00989-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/a2413f776034/fpls-08-00989-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/6f52ed7b7329/fpls-08-00989-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/f561df627863/fpls-08-00989-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/6cbd29ff2138/fpls-08-00989-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/1a183bb2c0dd/fpls-08-00989-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c3de/5462972/e992db920987/fpls-08-00989-g008.jpg

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