Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150000, China.
Aulin College, Northeast Forestry University, Harbin 150000, China.
Int J Mol Sci. 2023 Feb 7;24(4):3311. doi: 10.3390/ijms24043311.
(1) Background: The growth of plants is impacted by salinity and alkali, () is an ornamental plant with strong resistance to salinity and alkali, while the gene is helpful to fully understand the Saline-Alkali tolerance of . (2) Methods: Gene cloning, bioinformatics analysis, expression of fusion protein, determination of physiological indices of plant after Saline-Alkali stress, yeast two-hybrid screening, luciferase complementation assay, chromosome walking to obtain the promoter sequence, and then analyzed by PlantCARE. (3) Results: The gene was cloned and the fusion protein was purified. The transgenic plants had higher Saline-Alkali resistance than the wild type. A total of eighteen proteins interacting with LpPsbP were screened, and nine sites in the promoter sequence were analyzed. (4) Conclusion: Under Saline-Alkali or oxidative stress, will promote the expression of LpPsbP, which will then directly scavenge reactive oxygen species (ROS) in order to protect its photosystem II, reduce its damage, and thus improve the Saline-Alkali resistance of the plant. Moreover, according to some of the literature and the following experiments, two additional speculations are developed on the mechanisms of how two newly found objects, namely jasmonic acid (JA) and FoxO protein, could be involved in ROS scavenging processes were made.
(1) 背景：植物的生长受盐碱性的影响， （ ）是一种抗盐碱能力强的观赏植物，而 基因有助于充分了解 的耐盐碱性。(2) 方法：基因克隆、生物信息学分析、融合蛋白表达、盐碱性胁迫后植物生理指标测定、酵母双杂交筛选、荧光素酶互补测定、染色体步移获得启动子序列，然后通过 PlantCARE 进行分析。(3) 结果：克隆了 基因并纯化了融合蛋白。转基因植物的耐盐碱性高于野生型。共筛选到与 LpPsbP 相互作用的 18 种蛋白质，并对启动子序列的 9 个位点进行了分析。(4) 结论：在盐碱性或氧化胁迫下， 将促进 LpPsbP 的表达，进而直接清除活性氧（ROS），以保护其光系统 II，减轻其损伤，从而提高植物的耐盐碱性。此外，根据一些文献和后续实验，对两种新发现的对象——茉莉酸（JA）和 FoxO 蛋白——如何参与 ROS 清除过程的两种机制进行了推测。
