Clegg S, Pruckler J, Purcell B K
Infect Immun. 1985 Oct;50(1):338-40. doi: 10.1128/iai.50.1.338-340.1985.
Insertion mutants of recombinant plasmids encoding type 1 fimbriae of four genera of enteric bacteria were used to detect genetic complementation. After transformation by pairs of plasmids, double transformants were screened for their ability to express type 1 fimbriae. Complementation was observed between genes derived from the same genus but was absent with chimeric molecules carrying genetic information from two different genera. The results indicate that diffusible gene products of the fim cluster are necessary for phenotypic expression of type 1 fimbriae.
利用编码四种肠道细菌1型菌毛的重组质粒插入突变体来检测基因互补作用。在用成对质粒转化后，筛选双转化体表达1型菌毛的能力。观察到来自同一属的基因之间存在互补，但携带来自两个不同属遗传信息的嵌合分子不存在互补。结果表明，fim基因簇的可扩散基因产物对于1型菌毛的表型表达是必需的。
