Clegg S, Purcell B K, Pruckler J
Infect Immun. 1987 Feb;55(2):281-7. doi: 10.1128/iai.55.2.281-287.1987.
With a minicell system, the organization of genes encoding type 1 fimbriae of Salmonella typhimurium, Klebsiella pneumoniae, and Serratia marcescens was determined. In all cases multiple gene products were necessary for the phenotypic expression of fimbriae; thus fimbrial expression in these strains is similar to that in Escherichia coli. The type 1 fimbrial subunit gene was detected by the ability of its product to react with specific antiserum. At least six genes were found to be involved in the expression of type 1 fimbriae by S. typhimurium, and at least four genes constituted the fimbrial gene cluster of K. pneumoniae. In the case of S. marcescens, a minimum of three detectable polypeptides was required for the production of fimbriae. Also, a gene probe consisting in part of nucleotide sequences from the E. coli fimbrial subunit gene hybridized to a discrete DNA fragment derived from the plasmid encoding K. pneumoniae fimbriae. Such a fragment was assumed to contain a gene encoding the structural component of the type 1 fimbriae. Each of the three cloned systems encoded a number of polypeptides which varied in size; thus, the organization and molecular weight of fimbrial accessory proteins of each genus were not identical.
利用一个微小细胞系统，确定了编码鼠伤寒沙门氏菌、肺炎克雷伯菌和粘质沙雷氏菌1型菌毛的基因组织。在所有情况下，菌毛的表型表达都需要多种基因产物；因此，这些菌株中的菌毛表达与大肠杆菌中的相似。通过其产物与特异性抗血清反应的能力检测到1型菌毛亚基基因。发现鼠伤寒沙门氏菌中至少有六个基因参与1型菌毛的表达，肺炎克雷伯菌的菌毛基因簇至少由四个基因组成。就粘质沙雷氏菌而言，产生菌毛至少需要三种可检测的多肽。此外，一种部分由大肠杆菌菌毛亚基基因的核苷酸序列组成的基因探针与来自编码肺炎克雷伯菌菌毛的质粒的一个离散DNA片段杂交。假定这样一个片段包含一个编码1型菌毛结构成分的基因。这三个克隆系统各自编码了一些大小不同的多肽；因此，每个属的菌毛辅助蛋白的组织和分子量并不相同。
