Horii Takuro, Hatada Izuho
Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, 371-8512, Gunma, Japan.
Methods Mol Biol. 2017;1630:55-66. doi: 10.1007/978-1-4939-7128-2_5.
CRISPR/Cas enables rapid production of genome-edited animals. The Cas9/gRNA component can be introduced to fertilized eggs in several ways. Here, we provide an instructional guide for the generation of knockout mice by cytoplasmic injection using in vitro transcribed Cas9 and gRNA.
CRISPR/Cas技术能够快速培育出基因编辑动物。Cas9/gRNA组件可以通过多种方式导入受精卵。在此，我们提供一份使用体外转录的Cas9和gRNA通过细胞质注射来生成基因敲除小鼠的操作指南。
