Fujii Wataru
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Methods Mol Biol. 2017;1630:91-100. doi: 10.1007/978-1-4939-7128-2_8.
Knock-in mice are useful for evaluating endogenous gene expressions and functions in vivo. Instead of the conventional gene-targeting method using embryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-stranded oligodeoxynucleotide through the mouse zygote as an example of how to generate a knock-in mouse by genome editing.
敲入小鼠对于在体内评估内源性基因的表达和功能很有用。与使用胚胎干细胞的传统基因靶向方法不同，可以使用基因组编辑技术将外源DNA序列插入受精卵的目标位点。在本章中，我将以通过基因组编辑生成敲入小鼠为例，描述如何使用工程核酸酶和单链寡脱氧核苷酸通过小鼠受精卵生成表位标记小鼠。
