Department of Veterinary Medical Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Methods Mol Biol. 2023;2637:99-109. doi: 10.1007/978-1-0716-3016-7_8.
Knock-in mice are useful for evaluating endogenous gene expressions and functions in vivo. Instead of the conventional gene-targeting method using embryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome-editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an example of how to generate a knock-in mouse by genome editing.
敲入小鼠可用于评估体内内源性基因的表达和功能。与传统的胚胎干细胞基因靶向方法不同，利用基因组编辑技术，可将外源性 DNA 序列插入到受精卵的靶位点。本章以利用工程内切酶和单链寡脱氧核苷酸在受精卵中生成表位标记小鼠为例，描述了如何通过基因组编辑生成敲入小鼠。
