Watanabe Takahito, Noji Sumihare, Mito Taro
Graduate School of Bioscience and Bioindustry, Tokushima University, 2-1 Minami-Jyosanjima-cho, Tokushima, 770-8513, Japan.
Methods Mol Biol. 2017;1630:219-233. doi: 10.1007/978-1-4939-7128-2_18.
Hemimetabolous, or incompletely metamorphosing, insects are phylogenetically basal and include many beneficial and deleterious species. The cricket, Gryllus bimaculatus, is an emerging model for hemimetabolous insects, based on the success of RNA interference (RNAi)-based gene-functional analyses and transgenic technology. Taking advantage of genome editing technologies in this species would greatly promote functional genomics studies. Genome editing has proven to be an effective method for site-specific genome manipulation in various species. Here, we describe a protocol for genome editing including gene knockout and gene knockin in G. bimaculatus for functional genomics studies.
半变态昆虫，即不完全变态昆虫，在系统发育上处于基础地位，包括许多有益和有害物种。双斑蟋（Gryllus bimaculatus）是基于RNA干扰（RNAi）基因功能分析和转基因技术的成功，而新兴的半变态昆虫模式生物。利用该物种的基因组编辑技术将极大地促进功能基因组学研究。基因组编辑已被证明是在各种物种中进行位点特异性基因组操作的有效方法。在这里，我们描述了一种用于双斑蟋基因组编辑的方案，包括用于功能基因组学研究的基因敲除和基因敲入。
