Wowk Pryscilla Fanini, Zardo Maria Luisa, Miot Halisson Tesseroli, Goldenberg Samuel, Carvalho Paulo Costa, Mörking Patricia Alves
Instituto Carlos Chagas, Fundação Oswaldo Cruz, Fiocruz-PR, R. Prof. Algacyr Munhoz Mader, Curitiba - PR, Brazil.
Proteomics. 2017 Aug;17(15-16). doi: 10.1002/pmic.201600477. Epub 2017 Jul 18.
Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of EVs derived from T. gondii on the immune response and its relevance in a physiological context is unknown. Here we disclose the first proteomic profiling of T. gondii EVs compared to EVs isolated from a human foreskin fibroblast infected cell line cultured in a vesicle-free medium. Our results reveal a broad range of canonical exosomes proteins. Data are available via ProteomeXchange with the identifier PXD004895.
刚地弓形虫在全球感染广泛的宿主，包括人类和家畜，引发弓形虫病。最近，外泌体，即含有源自其原始细胞的核酸、蛋白质和脂质的小细胞外囊泡，与疾病保护相关联。来自刚地弓形虫的囊泡对免疫反应的影响及其在生理环境中的相关性尚不清楚。在这里，我们首次披露了与从在无囊泡培养基中培养的人包皮成纤维细胞感染细胞系中分离的囊泡相比，刚地弓形虫囊泡的蛋白质组学分析。我们的结果揭示了广泛的典型外泌体蛋白。数据可通过ProteomeXchange获得，标识符为PXD004895。
