理解基因型、生长年份和品种对突尼斯硬质小麦变应原性的影响。1. 面包师的哮喘病例。
Understanding the Effects of Genotype, Growing Year, and Breeding on Tunisian Durum Wheat Allergenicity. 1. The Baker's Asthma Case.
机构信息
Plant Protection and Improvement Laboratory, Center of Biotechnology of Sfax, University of Sfax , B.P 1177, Sfax 3018, Tunisia.
Food and Drug Department, University of Parma , Parco Area delle Scienze 27/A, 43124 Parma, Italy.
出版信息
J Agric Food Chem. 2017 Jul 19;65(28):5831-5836. doi: 10.1021/acs.jafc.7b02040. Epub 2017 Jul 3.
Baker's asthma is a serious airway disease triggered by wheat protein CM3 α-amylase/trypsin inhibitor. The purpose of the present study was to investigate the impact of genotype and crop year on allergen CM3 α-amylase/trypsin inhibitor associated with baker's asthma. A historical series of Tunisian durum wheat (100 accessions), derived from three crop years, was used to compare the amount of CM3 from landraces to advanced cultivars. CM3 protein quantification was assessed after an enzymatic cleavage of the soluble protein extracts on a UPLC/ESI-MS system, using a marker peptide for its quantification. Combined data analysis of variance revealed an important effect of genotype, crop year, and their interaction. The CM3 allergenic proteins were found to significantly vary among studied genotypes, as confirmed by genetic variability, coefficient of variance, heritability, and genetic advance.
贝克氏哮喘是一种严重的气道疾病，由小麦蛋白 CM3 α-淀粉酶/胰蛋白酶抑制剂引发。本研究旨在探讨基因型和作物年份对与贝克氏哮喘相关的过敏原 CM3 α-淀粉酶/胰蛋白酶抑制剂的影响。使用了一系列来自三个作物年份的突尼斯硬质小麦（100 个品系），以比较其与传统品种相比的 CM3 含量。在 UPLC/ESI-MS 系统上对可溶性蛋白提取物进行酶切后，使用标记肽进行 CM3 蛋白定量。方差分析的综合数据显示，基因型、作物年份及其相互作用具有重要影响。研究表明，CM3 过敏原蛋白在研究的基因型之间存在显著差异，这一点得到了遗传变异性、变异系数、遗传力和遗传进展的证实。
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