Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China; Key Laboratory of Microorganism Application and Risk Control (MARC) of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, Guangdong, PR China.
Environmental Simulation and Pollution Control State Key Joint Laboratory, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (SMARC), School of Environment, Tsinghua University, Beijing 100084, PR China.
Bioresour Technol. 2017 Nov;244(Pt 2):1254-1260. doi: 10.1016/j.biortech.2017.05.095. Epub 2017 May 17.
Heterotrophic cultivation of Chlorella pyrenoidosa based on straw substrate was proposed as a promising approach in this research. The straw pre-treated by ammonium sulfite method was enzymatically hydrolyzed for medium preparation. The highest intrinsic growth rate of C. pyrenoidosa reached to 0.097h in hydrolysate medium, which was quicker than that in glucose medium. Rising nitrogen concentration could significantly increase protein content and decrease lipid content in biomass, meanwhile fatty acids composition kept stable. The highest protein and lipid content in microalgal biomass reached to 62% and 32% under nitrogen excessive and deficient conditions, respectively. Over 40% of amino acids and fatty acids in biomass belonged to essential amino acids (EAA) and essential fatty acids (EFA), which were qualified for high-value uses. This research revealed the rapid biomass accumulation property of C. pyrenoidosa in straw hydrolysate medium and the effectiveness of nitrogen regulation to biomass composition at heterotrophic condition.
本研究提出了基于秸秆基质的栅藻异养培养方法，这是一种很有前途的方法。亚硫酸铵预处理的秸秆经酶解后用于制备培养基。在水解物培养基中，栅藻的最大固有生长率达到 0.097h，比在葡萄糖培养基中更快。氮浓度的升高可显著增加生物质中的蛋白质含量，降低脂质含量，同时保持脂肪酸组成的稳定。在氮过量和缺乏条件下，微藻生物质中的蛋白质和脂质含量最高分别达到 62%和 32%。生物质中超过 40%的氨基酸和脂肪酸属于必需氨基酸（EAA）和必需脂肪酸（EFA），可用于高价值用途。本研究揭示了栅藻在秸秆水解物培养基中的快速生物量积累特性，以及在异养条件下氮调节对生物质组成的有效性。
