Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, PR China.
Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, PR China.
Bioresour Technol. 2018 Feb;249:425-430. doi: 10.1016/j.biortech.2017.10.049. Epub 2017 Oct 14.
Glucose is normally used as the carbon source for heterotrophic cultivation of algal cells, whereas sucrose is difficult to be heterotrophicly utilized by them. In this study, a new co-culture system was developed through mixed culture of Chlorella pyrenoidosa with immobilized Saccharomyces cerevisiae in the dark to effectively obtain pure algal suspension using sucrose as only carbon source. In this system, a pure algal suspension with a concentration of 2.08g/L was obtained. The lipid content reached 29%, which was higher than that obtained in glucose contained system. In addition, the immobilized yeast beads were repeatedly used for at least three times. Through immobilization, the choice for the yeast strains that are able to hydrolyze sucrose was not limited by its product and pure algal suspension was efficiently obtained. This strategy may effectively decrease the cost of carbon source in the heterotrophic cultivation of microalgae.
葡萄糖通常被用作异养培养藻类细胞的碳源，而蔗糖则很难被它们异养利用。在这项研究中，通过在黑暗中混合培养小球藻和固定化酿酒酵母，开发了一种新的共培养系统，有效地利用蔗糖作为唯一碳源获得纯藻悬浮液。在该系统中，获得了浓度为 2.08g/L 的纯藻悬浮液。脂质含量达到 29%，高于含葡萄糖的系统中获得的含量。此外，固定化酵母珠至少重复使用了三次。通过固定化，对能够水解蔗糖的酵母菌株的选择不受其产物的限制，可以有效地获得纯藻悬浮液。该策略可以有效地降低微藻异养培养中碳源的成本。
