Wei Guo, Xia Du, Li-Li Wang, Hong Yan
College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150040, China.
Key Laboratory of Green Chemical Technology of College of Heilongjiang Province, Harbin, 150040, China.
Folia Microbiol (Praha). 2018 Jan;63(1):73-83. doi: 10.1007/s12223-017-0536-5. Epub 2017 Jun 23.
The microbial fuel cells (MFCs) are recognized to be highly effective for the biodegradation of phenol. For isolating the phenol-degrading bacteria, the sample containing 500 mg/L phenol was collected from the MFCs. The strain (WL027) was identified basing on the 16S rRNA gene analysis and phylogenetic analysis as Bacillus cereus. The effects of pH, temperature, concentrations of phenol, heavy metal ions, and salt on the growth of strain as well as the degradation of phenol have been carefully studied. The WL027-strain exhibited favorable tolerance for the metal cations including Cr, Co, Pb, and Cu with the concentration of 0.2 mg/L and NaCl solution with a high concentration of 30 g/L. In 41 h, 86.44% of 500 mg/L phenol has been degraded at the initial pH at 6 and the temperature of 30 °C. The strain was highly active electrogenesis bacteria and the coulombic efficiency reached 64.25%, which showed significant advantage on the efficient energy conversion. Therefore, due to the highly efficient degradation of phenol, WL027-strain could be used in the treatment of phenol-containing wastewater.
微生物燃料电池（MFCs）被认为对苯酚的生物降解非常有效。为了分离苯酚降解菌，从MFCs中采集了含500mg/L苯酚的样品。基于16S rRNA基因分析和系统发育分析，该菌株（WL027）被鉴定为蜡样芽孢杆菌。已仔细研究了pH值、温度、苯酚浓度、重金属离子和盐对该菌株生长以及苯酚降解的影响。WL027菌株对浓度为0.2mg/L的Cr、Co、Pb和Cu等金属阳离子以及浓度高达30g/L的NaCl溶液表现出良好的耐受性。在初始pH值为6、温度为30℃的条件下，41小时内500mg/L苯酚的降解率达到86.44%。该菌株是高效产电细菌，库仑效率达到64.25%，在高效能量转换方面具有显著优势。因此，由于对苯酚的高效降解，WL027菌株可用于含酚废水的处理。
