Sikora Dorota, Rocheleau Lynda, Brown Earl G, Pelchat Martin
Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada K1H 8M5.
Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada K1H 8M5.
Virology. 2017 Sep;509:167-177. doi: 10.1016/j.virol.2017.06.020. Epub 2017 Jun 22.
The influenza A virus RNA polymerase cleaves the 5' ends of host RNAs and uses these RNA fragments as primers for viral mRNA synthesis. We performed deep sequencing of the 5' host-derived ends of the eight viral mRNAs of influenza A/Puerto Rico/8/1934 (H1N1) virus in infected A549 cells, and compared the population to those of A/Hong Kong/1/1968 (H3N2) and A/WSN/1933 (H1N1). In the three strains, the viral RNAs target different populations of host RNAs. Host RNAs are cap-snatched based on their abundance, and we found that RNAs encoding proteins involved in metabolism are overrepresented in the cap-snatched populations. Because this overrepresentation could be a reflection of the host response early after infection, and thus of the increased availability of these transcripts, our results suggest that host RNAs are cap-snatched mainly based on their abundance without preferential targeting.
甲型流感病毒RNA聚合酶切割宿主RNA的5'端，并利用这些RNA片段作为病毒mRNA合成的引物。我们对感染的A549细胞中甲型流感病毒A/波多黎各/8/1934（H1N1）的八个病毒mRNA的5'宿主来源末端进行了深度测序，并将其群体与甲型流感病毒A/香港/1/1968（H3N2）和A/WSN/1933（H1N1）的群体进行了比较。在这三个毒株中，病毒RNA靶向不同的宿主RNA群体。宿主RNA根据其丰度进行帽抢夺，我们发现编码参与代谢的蛋白质的RNA在帽抢夺群体中过度富集。由于这种过度富集可能反映了感染后早期的宿主反应，从而反映了这些转录本可用性的增加，我们的结果表明，宿主RNA的帽抢夺主要基于其丰度，而没有优先靶向。
