Patrone F, Dallegri F, Brema F, Sacchetti C
Tumori. 1979 Feb 28;65(1):27-37. doi: 10.1177/030089167906500104.
Granulocyte function was studied in 9 patients with untreated, Ph1-positive chronic myelocytic leukemia (CML). The nitroblue tetrazolium reduction by stimulated granulocytes was impaired in all patients; 4 patients also had diminished phagocytosis and 2 others defective chemotaxis. In spite of this variety of polymorphonuclear (PMN) functional impairments, there is little evidence of increased susceptibility to infections in CML patients. This suggests that CML-PMN leucocytes (PMNs) may be successfully used for transfusion into neutropenic recipients, as previously reported. To evaluate the effects of irradiation and liquid storage on CML-PMNs, 5 of our patients were subjected to leukapheresis by continuous-flow centrifugation in the Aminco Celltrifuge, and granulocyte functional capacities were also evaluated on the cell-rich plasma immediately after collection and after short-term storage at 4 degrees C with or without irradiation (1500 rads). As evaluated by in vitro studies, granulocytes maintained, even after irradiation, functional activities similar to those found immediately after collection up to 24 h of storage at 4 degrees C and presented a moderate loss of function after 48 h. Chemotaxis appeared to be the most sensitive detector for cellular damage of stored leucocytes, irradiated and non-irradiated, so that it might be used for assessment of leucocyte function before transfusion.
对9例未经治疗的Ph1阳性慢性粒细胞白血病（CML）患者的粒细胞功能进行了研究。所有患者刺激粒细胞的硝基蓝四氮唑还原能力均受损；4例患者吞噬作用减弱，另外2例趋化性缺陷。尽管存在多种多形核（PMN）功能损害，但几乎没有证据表明CML患者对感染的易感性增加。这表明，如先前报道的那样，CML-PMN白细胞（PMNs）可成功用于输注给中性粒细胞减少的受者。为了评估照射和液体储存对CML-PMNs的影响，我们的5例患者在Aminco Celltrifuge中通过连续流动离心进行白细胞分离，并且在收集后以及在4℃下短期储存（有或无照射（1500拉德））后的富含细胞血浆上也评估了粒细胞功能能力。通过体外研究评估，粒细胞即使在照射后，在4℃储存长达24小时内仍保持与收集后立即发现的功能活性相似，并且在48小时后功能出现中度丧失。趋化性似乎是储存的照射和未照射白细胞细胞损伤的最敏感检测指标，因此它可用于输血前白细胞功能的评估。
