Determination of some anticonvulsants, antiarrhythmics, benzodiazepines, xanthines, paracetamol and chloramphenicol by reversed phase HPLC.

In this article assays are described for caffeine, theophylline, procainamide, N-acetylprocainamide, quinidine, dihydroquinidine, paracetamol, phenobarbital, phenytoin, carbamazepine, chloramphenicol, oxazepam, temazepam, diazepam, desmethyldiazepam, chlordiazepoxide, desmethylchlordiazepoxide and demoxepam using a uniform working procedure, five (slightly) different mobile phases and one HPLC system. Changing from one eluent to another is simple and a stable base-line is achieved within half an hour. Three of the five eluents are interchangeable and recycling of eluent causes no problems. Sample pretreatment is a single step extraction. Interferences can be overcome by changing the selectivity of the eluent by adjusting the tetrahydrofuran or triethylamine content. Furthermore it is shown how triethylamine can improve peak shapes of basic components and shorten their retention times.