Chavan Tejaswi, Ghadge Abhijit, Karandikar Manjiri, Pandit Vijaya, Ranjekar Prabhakar, Kulkarni Omkar, Kuvalekar Aniket, Mantri Nitin
Altern Ther Health Med. 2017 Jul;23(4):34-40.
Context • Guduchi Satwa is an Ayurvedic formulation prepared from Tinospora species. It has been used since ancient times to treat liver disorders. Objectives • The study intended to assess the hepatoprotective potential of Satwa prepared from 3 forms of Tinospora against alcohol-induced hepatotoxicity. Design • Male, albino Wistar rats were divided into 6 groups, with 6 rats each: 3 control groups-healthy controls, negative controls, and positive controls-and 3 intervention groups-Tinospora cordifolia, Tinospora sinensis, and Neem-Guduchi. Setting • The study was carried out at the Animal House facility of Bharati Vidyapeeth Deemed University's Medical College (Maharashtra, India). Intervention • Hepatotoxicity was induced by repeated dosing with alcohol for 15 d for all groups except for the healthy controls. To induce hepatotoxicity, the 5 groups received 1 mL of 30% alcohol PO per 100 g of body weight per day. The healthy controls and the negative controls received no hepatoprotective treatments. The other 4 groups received the dosing with alcohol 30 min after the hepatoprotective treatment, which they also received for 15 d: (1) positive controls-100 mg of silymarin per kg of body weight per day PO; (2) intervention group 1 (T cordifolia group)-200 mg of T cordifolia per kg of body weight per day PO; (3) intervention group 2 (T sinensis group)-200 mg of T sinensis per kg of body weight per day PO; and (4) intervention group 3 (Neem-Guduchi group)-200 mg of Neem-Guduchi per kg of body weight per day PO. Outcome Measures • Serum and liver tissue were used for biochemical analysis. Results • For the negative and positive control groups and the 3 intervention groups, the repeated dosing with alcohol produced elevations in the levels of liver-marker enzymes and changes in the lipid-profile status of the animals. Satwa from T cordifolia had a specific action in maintaining the lipid profile: total cholesterol, high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein. Improvement in the hepatic function, normalization of the lipid profile in the serum and liver, and improvements in the levels of antioxidant enzymes and oxidative-stress markers were observed in the animals treated with T sinensis Satwa. Neem-Guduchi Satwa was found to have a specific action in maintaining the lipid profile. The differential hepatoprotective effect of that Satwa was also evident from the liver histology. Conclusions • The data suggest that the 3 Satwa might be used in combination as a liver tonic that can help restore and strengthen the liver functions. The current study shows that the combination has the potential to be an effective liver tonic in animals. Scientific data from clinical trials of the 3 Satwa are not available. Systematic clinical trials are required that can yield information on their effects in humans.
背景 • 古杜奇散剂是一种由天仙藤属植物制成的阿育吠陀配方制剂。自古以来就被用于治疗肝脏疾病。
目的 • 本研究旨在评估由3种天仙藤属植物制成的散剂对酒精诱导的肝毒性的保肝潜力。
设计 • 将雄性白化Wistar大鼠分为6组，每组6只：3个对照组——健康对照组、阴性对照组和阳性对照组——以及3个干预组——心叶青牛胆、中华青牛胆和印楝 - 古杜奇。
地点 • 本研究在巴拉蒂维迪佩特被视为大学医学院（印度马哈拉施特拉邦）的动物房设施中进行。
干预 • 除健康对照组外，所有组均通过连续15天给予酒精来诱导肝毒性。为诱导肝毒性，5个组每天每100克体重经口给予1毫升30%的酒精。健康对照组和阴性对照组未接受保肝治疗。其他4个组在接受保肝治疗30分钟后给予酒精，持续15天：(1)阳性对照组——每天每千克体重经口给予100毫克水飞蓟宾；(2)干预组1（心叶青牛胆组）——每天每千克体重经口给予200毫克心叶青牛胆；(3)干预组2（中华青牛胆组）——每天每千克体重经口给予200毫克中华青牛胆；(4)干预组3（印楝 - 古杜奇组）——每天每千克体重经口给予200毫克印楝 - 古杜奇。
观察指标 • 采集血清和肝组织进行生化分析。
结果 • 对于阴性和阳性对照组以及3个干预组，连续给予酒精导致动物肝脏标志物酶水平升高以及血脂状况改变。心叶青牛胆制成的散剂在维持血脂方面有特定作用：总胆固醇、高密度脂蛋白、低密度脂蛋白和极低密度脂蛋白。用中华青牛胆散剂治疗的动物肝脏功能得到改善，血清和肝脏中的血脂恢复正常，抗氧化酶和氧化应激标志物水平也有所改善。发现印楝 - 古杜奇散剂在维持血脂方面有特定作用。从肝脏组织学也可明显看出该散剂不同的保肝作用。
结论 • 数据表明，这3种散剂可能联合用作肝脏滋补剂，有助于恢复和增强肝脏功能。当前研究表明，该组合在动物中有可能成为一种有效的肝脏滋补剂。目前尚无这3种散剂的临床试验科学数据。需要进行系统的临床试验，以获取它们对人类影响的信息。
