Skowron Krzysztof, Jakubicz Agnieszka, Budzyńska Anna, Kaczmarek Agnieszka, Grudlewska Katarzyna, Reśliński Adrian, Gospodarek-Komkowska Eugenia
Department of Microbiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Poland
Department of General Surgery and Transplantology, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Poland
Rocz Panstw Zakl Hig. 2017;68(2):191-197.
Microbiological purity of cosmetics provides safety of users during their use, prevents physicochemical changes of a preparation, infections and diseases of the skin.
The aim of this study was to assess the level of microbiological contamination of cosmetics used by one person and by several people and cosmetics after their expiry date in relations to standards for marketed cosmetics, ensuring safety of their use.
This study was conducted using 55 samples representing 19 types of cosmetics, divided into three groups: used by one person, used by several people and after the expiry date. In cosmetic samples the general numbers of aerobic mesophilic bacteria were determined with the spread plate method on tryptic-soy agar. The presence of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans were also checked.
The number of aerobic mesophylic bacteria in the tested cosmetics ranged from the level below the method detectability to 1.3×107 cfu/g or ml. The presence of Staphylococcus spp. was found in 11 (20.0%) tested cosmetic samples and of P. aeruginosa in one tested preparation. Yeasts C. albicans were not detected, whereas contamination with fungi Aspergillus spp. and Penicillium spp. ranging from 0.5×101 to 1.5×101 cfu/g or ml was recorded in four cosmetics. The level of microbiological contamination of cosmetics used by several people was higher than that of cosmetics used by one person. Cosmetics after the expiry date showed the highest microbiological contamination.
The number of users of cosmetic and it expiry date exceeding influenced the level of microbial contamination of preparations.
化妆品的微生物纯度可确保使用者在使用过程中的安全，防止制剂发生物理化学变化、皮肤感染和疾病。
本研究旨在评估单人使用的化妆品、多人使用的化妆品以及过期化妆品的微生物污染水平，与市售化妆品标准进行对比，以确保其使用安全。
本研究使用了代表19种化妆品的55个样本，分为三组：单人使用的、多人使用的和过期的。在化妆品样本中，采用平板涂布法在胰蛋白胨大豆琼脂上测定需氧嗜温菌的总数。还检查了金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌的存在情况。
受试化妆品中需氧嗜温菌的数量范围从低于方法可检测水平到1.3×10⁷ cfu/g或ml。在11个（20.0%）受试化妆品样本中发现了葡萄球菌属，在一个受试制剂中发现了铜绿假单胞菌。未检测到白色念珠菌酵母，而在四种化妆品中记录到曲霉属和青霉属真菌的污染，范围为0.5×10¹至1.5×10¹ cfu/g或ml。多人使用的化妆品的微生物污染水平高于单人使用的化妆品。过期化妆品的微生物污染程度最高。
化妆品的使用人数及其过期时间会影响制剂的微生物污染水平。
