Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, South Korea.
Research Institute, Korea District Heating Corp., 186 Bundang-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, South Korea.
Bioresour Technol. 2017 Nov;244(Pt 2):1235-1244. doi: 10.1016/j.biortech.2017.05.147. Epub 2017 May 26.
The aim of this study was to develop the economic and effective buffer system for microalgae mass cultivation using industrial flue gas. Due to the continuous flue gas supplement, culture media acidified, therefore cell growth inhibited. Although buffering agent was added, this result increase in cost for overall culture process. Therefore combined buffer system of bicarbonate and phosphate (BP) for large-scale use was investigated. The bicarbonate buffer system generated from CO dissolution, additionally phosphate buffer system improves the buffer performance under the continuous CO supplementation from flue gas. The microalgae Haematococcus pluvialis was cultivated under autotrophic outdoor conditions using these buffer solutions. As a result, the autotrophic BP buffer system enhanced the biomass and astaxanthin productivity of H. pluvialis to 105% and 103%, respectively. The results confirm that the BP buffer system reduces the cost of microalgal CO conversion process, particularly for the outdoor mass cultivation.
本研究旨在开发经济有效的微藻大规模培养缓冲体系,以利用工业烟道气。由于不断补充烟道气,培养基酸化,从而抑制细胞生长。尽管添加了缓冲剂,但这会增加整个培养过程的成本。因此,研究了碳酸氢盐和磷酸盐(BP)的组合缓冲体系,以实现大规模应用。碳酸氢盐缓冲体系是由 CO2 溶解产生的,此外,磷酸盐缓冲体系可改善在连续补充烟道气 CO2 条件下的缓冲性能。使用这些缓冲溶液,在自养户外条件下培养了雨生红球藻。结果表明,自养 BP 缓冲体系使 H. pluvialis 的生物量和虾青素生产力分别提高了 105%和 103%。结果证实,BP 缓冲体系降低了微藻 CO2 转化过程的成本,特别是对于户外大规模培养。