Yu Byung Sun, Hong Min Eui, Sung Young Joon, Choi Hong Il, Chang Won Seok, Kwak Ho Seok, Sim Sang Jun
Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul 02841, South Korea.
Research Institute, Korea District Heating Corp., 92, Gigok-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17099, South Korea.
Bioresour Technol. 2021 Jul;332:125121. doi: 10.1016/j.biortech.2021.125121. Epub 2021 Apr 6.
Currently, there is a lack of an efficient, environmentally-benign and sustainable industrial decontamination strategy to steadily achieve improved astaxanthin production from Haematococcus pluvialis under large-scale outdoor conditions. Here, this study demonstrates for the first time that a CaCO biomineralization-based decontamination strategy (CBDS) is highly efficient in selectively eliminating algicidal microorganisms, such as bacteria and fungi, during large-scale H. pluvialis cultivation under autotrophic and mixotrophic conditions, thereby augmenting the astaxanthin productivity. Under outdoor AT and MT conditions, the average astaxanthin productivity of H. pluvialis using CBDS in a closed photobioreactor system was substantially increased by 14.85- (1.19 mg L d) and 13.65-fold (2.43 mg L d), respectively, compared to the contaminated H. pluvialis cultures. Given the exponentially increasing demand of astaxanthin, a natural anti-viral, anti-inflammatory, and antioxidant drug, CBDS will be a technology of interest in H. pluvialis-based commercial astaxanthin production which has been hindered by the serious biological contaminations.
目前，缺乏一种高效、环境友好且可持续的工业去污策略，以在大规模户外条件下稳步提高雨生红球藻虾青素的产量。在此，本研究首次证明，基于碳酸钙生物矿化的去污策略（CBDS）在自养和混合营养条件下大规模培养雨生红球藻期间，在选择性消除诸如细菌和真菌等杀藻微生物方面具有高效性，从而提高了虾青素的生产力。在户外自养和混合营养条件下，与受污染的雨生红球藻培养物相比，在封闭光生物反应器系统中使用CBDS的雨生红球藻的平均虾青素生产力分别大幅提高了14.85倍（1.19毫克/升·天）和13.65倍（2.43毫克/升·天）。鉴于虾青素（一种天然抗病毒、抗炎和抗氧化药物）的需求呈指数级增长，CBDS将成为基于雨生红球藻的商业虾青素生产中一项受关注的技术，该生产一直受到严重生物污染的阻碍。
