Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.
Department of Life Science, Hanyang University, 206, Wangsimni-ro, Seongbuk-gu, Seoul, 04763, Republic of Korea.
Nat Commun. 2021 Oct 18;12(1):6049. doi: 10.1038/s41467-021-26325-5.
Microalgae can accumulate various carbon-neutral products, but their real-world applications are hindered by their CO susceptibility. Herein, the transcriptomic changes in a model microalga, Chlamydomonas reinhardtii, in a high-CO milieu (20%) are evaluated. The primary toxicity mechanism consists of aberrantly low expression of plasma membrane H-ATPases (PMAs) accompanied by intracellular acidification. Our results demonstrate that the expression of a universally expressible PMA in wild-type strains makes them capable of not only thriving in acidity levels that they usually cannot survive but also exhibiting 3.2-fold increased photoautotrophic production against high CO via maintenance of a higher cytoplasmic pH. A proof-of-concept experiment involving cultivation with toxic flue gas (13 vol% CO, 20 ppm NO, and 32 ppm SO) shows that the production of CO-based bioproducts by the strain is doubled compared with that by the wild-type, implying that this strategy potentially enables the microalgal valorization of CO in industrial exhaust.
微藻可以积累各种碳中性产品，但由于其对 CO 的敏感性，其实际应用受到阻碍。在此，评估了模型微藻莱茵衣藻在高 CO 环境（20%）中的转录组变化。主要毒性机制包括质膜 H+-ATPase（PMAs）异常低表达，伴随细胞内酸化。我们的结果表明，在野生型菌株中表达普遍表达的 PMA 不仅使它们能够在通常无法生存的酸度水平下茁壮成长，而且通过维持更高的细胞质 pH 值，使其在高 CO 下的光自养产量增加了 3.2 倍。涉及有毒烟道气（13%CO、20 ppmNO 和 32 ppmSO）培养的概念验证实验表明，与野生型相比，该菌株生产的 CO 基生物产品增加了一倍，这意味着该策略有可能使微藻能够利用工业废气中的 CO。
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