Guo Yuanheng, Zhao Qingsheng, Cao Lili, Zhao Bing
Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Division of Biorefinery Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
J Ethnopharmacol. 2017 Aug 17;208:1-7. doi: 10.1016/j.jep.2017.06.033. Epub 2017 Jun 21.
Gan Kang Yuan (GKY) is a compound medicine formulated on the basis of traditional Chinese medicine (TCM). It was composed of Herba Cistanchis (Roucongrong), Radix Puerariae (Gegen), Radix Astragali (Huangqi), Fructus Schisandrae (Wuweizi) and Radix Glycyrrhizae (Gancao).
The purpose of this study is to research the hepatoprotective effect of GKY against liver injury induced by alcohol, and to elucidate the mechanism of hepatopretective effect.
Hepatoprotective activity of GKY was researched both in vivo and vitro. In vitro, effect of GKY on the survival rates of HepG2 cells were assessed. In vivo research, ICR mice were oral administrated with alcohol (Er Guo-tou white spirit, 56%, 6mL/kg, once per day) for 31 days to establish liver injury model. Meanwhile, positive group or experimental groups were treated with bicyclol (300mg/kg) or GKY (200, 600, 1800mg/kg). Serological indexes including aspartate and alanine transaminases (AST, ALT), γ-glutamyl transpeptidase (γ-GTP), total bilirubin (TBil), total cholesterol (TCHO) and serum triglyceride (STG) were estimated. Hepatic indicators including superoxide dismutase (SOD), glutathione S-transferase (GST), glutathione (GSH), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and liver triglyceride (LTG) were analyzed. Histopathologic changes of liver tissue were observed.
The survival rates of HepG2 cell were observably promoted by GKY. Alcoholic treatment drastically altered the serum indexes and liver indicators of model animals, while these alteration were significantly ameliorated by GKY (p < 0.05, 0.01 or 0.001) in experimental group. The microvesicular steatosis and necrosis in hepatic histopathology induced by alcoholic treatment also were notably attenuated by GKY administration.
These findings indicated that GKY possessed hepatoprotective property against liver injury induced by ethanol. GKY significantly promoted activities of relative enzymes and suppressed the contents of MDA and LTG, which might be the mechanism of hepatoprotective effect of GKY.
肝康源（GKY）是一种基于传统中药（TCM）配制的复方药物。它由肉苁蓉、葛根、黄芪、五味子和甘草组成。
本研究旨在探讨肝康源对酒精诱导的肝损伤的保肝作用，并阐明其保肝作用机制。
在体内和体外研究肝康源的保肝活性。体外，评估肝康源对HepG2细胞存活率的影响。体内研究中，将ICR小鼠用酒精（二锅头白酒，56%，6mL/kg，每天一次）灌胃31天以建立肝损伤模型。同时，阳性组或实验组分别用双环醇（300mg/kg）或肝康源（200、600、1800mg/kg）治疗。检测血清学指标，包括天冬氨酸转氨酶和丙氨酸转氨酶（AST、ALT）、γ-谷氨酰转肽酶（γ-GTP）、总胆红素（TBil）、总胆固醇（TCHO）和血清甘油三酯（STG）。分析肝脏指标，包括超氧化物歧化酶（SOD）、谷胱甘肽S-转移酶（GST）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）和肝脏甘油三酯（LTG）。观察肝组织的组织病理学变化。
肝康源可显著提高HepG2细胞的存活率。酒精处理显著改变了模型动物的血清指标和肝脏指标，而实验组中肝康源显著改善了这些改变（p<0.05、0.01或0.001）。酒精处理诱导的肝组织病理学中的微泡性脂肪变性和坏死也因肝康源给药而显著减轻。
这些结果表明，肝康源对乙醇诱导的肝损伤具有保肝作用。肝康源显著提高相关酶的活性，抑制MDA和LTG的含量，这可能是肝康源保肝作用的机制。
